A Comparative Study Of Exhaled Gas Components Before And After Chemotherapy In Non-small Cell Lung Cancer

Objective:To evaluate the changes of volatile organic compounds(VOCs)in the breath as therapeutic effect index,we used the extractive electrospray ionization(EESI)source coupled with LTQ-XL mass spectrometer(EESI-MS)to detect the components and levels of VOCs of advanced non-small cell lung cancer(NSCLC)before and after chemotherapy.Methods:24 advanced NSLC patients and 29 healthy controls respectively came from the the respiratory department and health department in the First Affiliated Hospital of Nanchang University,Jiangxi Province during November 2015 to September 2016.We collected the exhaled gas of NSCLC patients and controls and used the EESI-MS in combination with principal component analysis(PCA)to detect and analyze VOCs existing in the breath,and then evaluated the changes of VOCs for NSCLC patients before and after chemotherapy.Results:(1)There were significant differences in the compounds of the breath between controls and NSCLC patients before and after chemotherapy.(2)By PCA and statistical method,We compared the gas chromatographic-mass spectrometric(GC-MS)signal between controls and NSCLC patients and found that4 kinds of VOCs(butadiene,tetrahydrobiopterin,C8H18O2N2 and C10H16O2N2)in the breath of NSCLC patients were different from the controls.(3)By PCA and statistical method,we compared the gas chromatographic-mass spectrometric(GC-MS)signal of NSCLC patients before and after chemotherapy and found that 3 kinds of VOCs(butadiene,C8H18O2N2 and C10H16O2N2)in the breath of NSCLC patients accepting chemotherapy were different from themselves before chemotherapy.Conclusions:(1)The 4 kinds of VOCs(butadiene,tetrahydrobiopterin,C8H18O2N2 and C10H16O2N2)is difference between patients with lung cancer and healthy people.(2)The 3 kinds of VOCs(butadiene,C8H18O2N2 and C10H16O2N2)is difference between patients with lung cancer before and after chemotherapy.