Impact Of SNP On Prognosis Of Patients After Matched Unrelated Allo-HSCT

Part ? Impact of IL10-592 locus AA genotype on the prognosis of patientsafter HLA-10/10 matched unrelated allo-HSCTObjectives To explore the impact of IL10-592(rs1800872)single nucleic acid polymorphism(SNP)on the prognosis of HLA matched unrelated hematopoietic stem cell transplantation(HSCT).Methods The SNP of IL10-592 in 104 recipient-donor pairs and 100 healthy volunteers was analyzed with sequence based typing(SBT).Results When the genotype of IL10-592 in recipients and donors matched,AA/AA genotype had higher incidence of?~?a GVHD than AC/AC or CC/CC genotype(47.1%,3.7%,0,P=0.002).When the genotype of IL10-592 recipients and donors mismatched,recipients with AC genotype or donors with AA genotype,there was significant different incidence of ?~?a GVHD among donors or recipients with different genotype(P=0.046,P=0.041).The recipients with AA genotype had higher incidence of ?~?a GVHD than with AC or CC genotype(27.8%vs10.2%,11.1%;P=0.072),and higher incidence of intestinal a GVHD(22.2%vs5.1%,11.1%;P=0.040),lower incidence of 2-year OS(48.2%vs75.1%,85.7%;P=0.002),lower incidence of 2 year DFS(48.5%vs66.3%,76.2%;P=0.045).Patients had higher incidence of ? ~ ? a GVHD with donors of AA genotype than with donors of AC or CC genotype(26.5%vs8.9%,0;P=0.024),and higher incidence of intestinal a GVHD(20.4%vs4.4%,0;P=0.026).In multivariate analysis,the genotype of IL10-592 AA in recipients and donors had increased risk of ?~?a GVHD(OR=3.3,P=0.049;OR=3.9,P=0.043).There were no statistical differences on the incidence of c GVHD and relapse.Conclusion In HLA-10/10 matched unrelated HSCT,the presence of IL10-592 AA genotype in recipients and/or donors increased the risk of ?-? a GVHD and decreasedthe probability of 2-year OS,2-year DFS.Part ? Impact of rs9277534 genotype in DPB1 on the prognosis of patientsafter HLA-10/10 matched unrelated allo-HSCTObjectives To explore the impact of rs9277534 SNP on the prognosis of HLA matched unrelated hematopoietic stem cell transplantation(HSCT).Methods The polymorphism of rs9277534 in 105 recipient-donor pairs and 100 healthy volunteers was analyzed with sequence based typing(SBT).The correlation of rs9277534 genotype with prognosis of patients after HSCT was analyzed.Results The frequencies of rs9277534 AA,AG,GG genotype in patients,donors and volunteers showed no significant difference.When the genotype of rs9277534 in donors were GG,recipients with GG genotype had higher incidence of ?~?a GVHD than AA or AG genotype(57.1% vs 40.0%,0%;P=0.019)When the genotype of rs9277534 in donors were AA,recipients with AA genotype had higher incidence of relapse than AG or GG genotype(50% vs 6.7%,0%;P=0.023).The recipients with AG genotype had highest incidence of 2-year OS than AA and GG genotype(58.2% vs 78.5%,57.4%;P=0.016).Conclusion In HLA-10/10 matched unrelated HSCT,when the composition genotype of rs9277534 in recipients and donors was GG/GG,significantly increased the risk of ?-? a GVHD,when the genotype composition was AA/AA,there was increased incidence of relapse.A heterozygous genotype of in recipients increased the incidence of survival.Part ? Impact of rs987870 genotype in DPA1 on the prognosis of patientsafter HLA-10/10 matched unrelated allo-HSCTObjectives To explore the impact of rs987870 SNP on the prognosis of HLA matched unrelated hematopoietic stem cell transplantation(HSCT).Methods The polymorphism of rs987870 in 104 recipient-donor pairs and 90 healthy volunteers was analyzed with sequence based typing(SBT).The correlation of rs9277534 genotype with prognosis of patients after HSCT was analyzed.Results The frequencies of rs987870 TT,TC,CC genotype in patients,donors and volunteers showed no significant difference.When the genotype of rs987870 in recipients was TT and TC+CC,the incidence of ?~? a GVHD was 18.2% and 11.1% respectively(P=0.581);When the genotype of rs987870 in donors was TT and TC+CC,the incidence of ?~? a GVHD of recipients was 16.0% and 17.2% respectively(P>0.05);When the genotype of rs987870 in donors was TC+CC,recipients with TC+CC genotype had increased incidence of c GVHD,not significantly.(36.4%,62.5%;P=0.252).There was no relationship between the genotype of rs987870 in recipients or donors and relapse,OS and DFS.Conclusion In HLA-10/10 matched unrelated HSCT,When the genotype of rs987870 in donors was TC+CC,recipients with TC+CC genotype inclined to have increased incidence of c GVHD,not statistic significantly.