| ObjectiveTo investigate the changes and clinical significance of Paneth cells in the pathogenesis of experimental terminal enteritis.MethodsA total of 60 male SD rats in a cleanness grade were selected in the experiment,body weight of the rat was in the range of 250 g to 300 g.Included SD rats were randomly divided into the following three groups:group A,model group;group B,suture group;and group C,control group,with equal number of rats in each group.Rats in the three groups received different experimental treatments.In the model group,the side-to-side anastomosis was performed after the 1cm incision was made in the initial region of the ileum and colon 3cm from the ileocecal valve,to establish colon-ileum reflux model.Following a 1cm incision in the initial region of the ileum and colon 3cm from the ileocecal valve,no intervention was made in the suture group,after that,surgical suture was done and no reflux model was established.In the control group,only the same dose of anesthetics was given as other groups,no surgical treatment was madeafter abdominal anaesthesia.The three groups of SD rats were placed in the same environment after different treatment,and were given the same feeding and nursing.Subsequently,2 weeks and 8 weeks after operation,mucosal tissues 1-3cm from the distal ileum were randomly selected from10 SD rats in each group.Mucosa tissues of terminal ileum were observed by gross,and then divided into two parts,one was used for HE staining and cut into slices to observe the terminal ileum mucosa under the microscope,the other was applied for the detection of the number of SC and lysozyme positive cells secreted by the cells using immunohistochemical assay.Results1.Macroscopic and microscopic observation results of mucosa tissues of terminal ileum in SD rats of the three groups showed that: 2weeks after operation,acute inflammation was observed by naked eye concerning mucosa tissues of terminal ileum in SD rats of the model group and the suture group,neutrophil infiltration was mainly inhibited under microscopic observation,inflammation was much obvious in the model group,the difference was statistically significant(P<0.05).Meanwhile,8 weeks after operation,chronic inflammation was observed by naked eye in SD rats of the model group,infiltration of lymphocyte and plasmocyte was mainly seen under microscopic observation;yet there was no obvious inflammatory change in the mucosa tissues ofterminal ileum of SD rats in the suture group and the control group by macroscopic and microscopic observation,the difference was significant(P<0.05);but there was no statistical difference in the comparison between the suture group and the control group(P>0.05).2.Detection results of SC positive cells of Paneth cells in SD rats from the three groups revealed that: 2 weeks after operation,the number of SC positive cells was more in the suture group and the model group compared with the control group,and the difference was statistically significant(P<0.05),however,there was no significant difference between the suture group and the model group(P>0.05).Furthermore,8weeks after operation,the number of SC positive cells decreased in the mucosa tissues of terminal ileum in the suture group,but there was no significant difference compared with the control group,the difference was not statistically significant(P>0.05).The number of SC positive cells in the mucosa tissues of terminal ileum of the model group was higher than that of the suture group and the control group.However,compared with the results of 2 weeks after surgery,the difference was statistically significant between the suture group and the control group(P<0.05).3.Detection results of lysozyme positive cells in SD rats of three groups indicated that: 2 weeks after operation,compared with the control group,the number of lysozyme positive cells in the mucosa tissues of terminal ileum increased in the model group and the suture group,thedifference was statistically significant(P<0.05).In addition,the number of lysozyme positive cells decreased 8 weeks after operation when compared to that of 2 weeks after operation;nevertheless,compared with the suture group and the control group,the amount of lysozyme positive cells in the mucosa tissues of terminal ileum was higher in the model group,with statistical difference(P<0.05).Furthermore,there was no statistical difference in the comparison of the number of lysozyme positive cells secreted by Paneth cells in rats’ mucosa tissues of terminal ileum between the suture group and the control group(P>0.05).4.Correlation analysis of SC positive cells and inflammatory degree in SD rats through each period documented that: from the second weeks to the eighth weeks after operation,the number of SC positive cells in the model group increased with the increase of the inflammation of the terminal ileum mucosa in SD rats’ mucosa tissues of terminal ileum.At2 weeks,there was a positive correlation between the inflammation score and the number of SC positive cells(R=0.784,P<0.05),besides,a positive correlation was also found between the inflammation score and the number of SC positive cells at 8 weeks(R=0.684,P<0.05).5.Correlation between the number of lysozyme positive cells and inflammatory degree in experimental terminal ileum(ETI)of SD rats through each period revealed that: from the second weeks to the eighth weeks after operation,the number of lysozyme positive cells in the modelgroup increased with the aggravation of the inflammation of the terminal ileum mucosa in SD rats’ mucosa tissues of terminal ileum.There were positive correlations between the inflammation score and the number of SC positive cells at both 2 weeks and 8 weeks(R=0.744 and R=0.868,both P<0.05).ConclusionsThere is an elevation of Paneth cells in the acute and chronic stage of terminal ileitis,and increases with the degree of inflammation.It is speculated that the changes of intestinal microecology and the intestinal humoral immune system may be involved in the pathogenesis of the disease. |
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