The Effect Of Dexmedetomidine On The Proliferation And Apoptosis Of Neuroblastoma

objectives The aim of the study was to explore the effect of dexmedetomidine on the proliferation and apoptosis of neuroblas toma.Methods 1、The SH-SY5 Y cells were respectively treated wit h 0、0.1uM、1uM、10u M dexmedetomidine and screened optimal co ncentration;dexmedetomidine dealed with the SH-SY5 Y cells about0、6h、12h、24h respectively and screened optimal time;The SH-S Y5 Y cells were respectively treated with 0、100uM、150uM、200uM yohimbine and screened optimal concentration;yohimbine dealed w ith the SH-SY5 Y cells about 0、6h、12h、24h respectively and scre ened optimal time.Cell proliferation was evaluated by MTT assays.2、In optimal time,the SH-SY5 Y cells,dividing into control group、dexmedetomidine group and yohimbine group were incubated with o ptimal concentration.The effect of dexmedetomidine and yohimbine on apoptosis was assessed by flow cytometry.3、q-PCR analyzed t he mRNA expression of α2-adrenergic receptor of SH-SY5 Y cells.Results MTT: Cell proliferation rate increased as dexmedetomidine’s concentration increased and dexmedetomidine’s optimal con centration was 1uM;with time increased,Cell proliferation rate incre ased and dexmedetomidine’s optimal time was 12h;Cell proliferation rate decreased as yohimbine’s concentration increased and yohimbi ne’s optimal concentration was 200uM;with time increased,Cell pro liferation rate decreased and yohimbine’s optimal time was 12h;FC M:The apoptosis rate of dexmedetomidine group was lower than th at of control group;The apoptosis rate of yohimbine group was hi gher than that of control group.q-PCR:SH-SY5 Y cells expressed α2-adrenergic receptors.Conclution Dexmedetomidine could enhance SH-SY5 Y proliferation and inhibited its apoptosis.SH-SY5 Y cells expressed α2-adrenergic receptors.