Effects Of Silencing CIP2A Gene On The Expression Of C-Myc Protein And Proliferation Of Gastric Cancer Cell Line MGC803

Objectives :The effect of CIP2 A expression on the expression of c-myc protein and the proliferation of gastric cancer cells was observed by silencing MGC-803 gene in gastric cancer cell line CIP2 A.Methods :1.The expression of CIP2 A protein in normal gastric mucosa,gastric cancer cell line MGC803 and gastric cancer cell line SGC7901 was detected by Western blot.2.Two plasmids with different CIP2 A interference sequences were constructed to infect MGC-803 cells,The experimental group was divided into blank control group,CIP2A-shRNA1 infection group,CIP2A-sh RNA2 infection group,empty plasmid transfection group,The expression of CIP2 A protein and the expression of c-myc protein in each group were detected by Western blot.The proliferation of gastric cancer cell lines was detected by MTT in four groups.Finally,The experimental data were statistically analyzed.Results: 1.Western blot was used to detect the expression of CIP2 A protein in normal gastric mucosa,gastric cancer cell line MGC803 and gastric cancer cell line SGC7901,and the results showed that the CIP2 A protein in normal gastric mucosa cells showed low expression,The expression of CIP2 A protein in gastric cancer cell line MGC803 and gastric cancer cell line SGC7901 was high,the difference was statistically significant(p<0.05).2.Successfully constructed two CIP2A-shRNA sequences3.Plasmid mediated CIP2A-shRNA1 and plasmid mediated CIP2A-shRNA2 were used to infect gastric cancer cell line MGC-803,and the green fluorescence of the cells was detected showed that the plasmid successfully infected MGC803 Cells,transfection rate was about 80%~90%.4.The experimental group was divided into 4 groups,and the gastric cancer cells of the 4 groups were detected by Western blot,the results showed that the expression level of CIP2A-shRNA1 interference group was lower than that of untreated control group,and the difference was statistically significant(p<0.05);At the same time,the expression level of CIP2A-shRNA interference group was lower than the empty plasmid transfection group,the difference was statistically significant(p<0.05);Compared the blank control group with the MGC803 Cells transfected with empty vector group had no significant difference(p>0.05).5.MTT colorimetric test results showed,the cell proliferation inhibition rate of CIP2A-shRNA1 interference group’s the twenty-fourth hour and the forty-eighth hour was 26.66%,40.77%;and the cell proliferation inhibition rate of CIP2A-shRNA1 interference group’s the twenty-fourth hour and the forty-eighth hour was 25.22%,40.42%,compared with untreated control group,the proliferation of the two groups was significantly inhibited,the difference was statistically significant(p<0.05);At the same time,the cell proliferation inhibition rates of empty vector transfected group’s the twenty-fourth hour and the forty-eighth hour were 2.60%,7.77%,there was statistical significance in cip2a-shrna interference group,gastric cancer cell proliferation inhibition rate compared with the empty plasmid transfected group compared the difference(p<0.05).Conclusions:1.The expression of CIP2 A protein in gastric cancer cell lines was high.2.Downregulation of c-myc protein expression after silencing CIP2 A gene.3.Silencing CIP2 A gene inhibits proliferation of gastric cancer cell line MGC803.