Study On Spatial Distribution Of Breast Cancer Cells Regulated By Topological Microenvironment Of Grooves

Cancer is currently a prevalent and high-mortality disease.Medically,malignant tumors on epithelial tissues are generally called cancers.Cancer metastasis is the migration of primary tumor cells into the blood or lymphatic system resulting in new tumor community formation.Cell migration plays an important role in this complex process of cancer metastasis and is the basis for the development and pathology of multicellular organisms.In general,the actin cytoskeleton controls cell migration by changing cell shape.Since cancer cells migrate through the body in a heterogeneous microenvironment and have a variety of migratory mechanisms,there are considerable challenges for clinical research.The current popular method is to study cell migration by using a known physical and chemical composition and simulate the limited microenvironment in vivo.A large number of studies have shown that the micro-nanoscale topological structure plays an important role in the regulation of cellular behaviors by the physical microenvironment.With the continuous advancement of research,it is well known that most of the behavior of cells is gradually induced by biological,chemical and physical interactions.With the use of advanced Micro-Nanofabrication technology and cell-biologically compatible polymer materials,cell-like microenvironments of different scales and morphologies can be fabricated to regulate the cell’s behavior.It is of great significance in the study of cancer metastasis.In this paper,cell-behavior is regulated by designing and fabricating different scales and shapes and using polymer materials with good cell biocompatibility(polyacrylamide gel and polydimethylsiloxane).For MDA-MB-231 human breast cancer cells,the study of the geometric parameters of the polyacrylamide gel groove substrate for the best regulation of cell space distribution;and then for the relatively optimal parameters of the substrate,there is some analysis about which intrinsic factors can affect the cell spatial distribution(substrate depth,cell density,difference in oxygen concentration,cell speed of migration,and intercellular connectivity);for SUM 159 human breast cancer cells and MCF10A human normal breast epithelial cells,The spatial distribution of the cells on the polydimethylsiloxane substrate was studied based on the depth and the width of the substrate was constant,while the width of the bottom changed.Since the close contact of multiple cells in vivo,the co-culture of the two cells was used to investigate the effect of the substrate on cell-behavior under mixing conditions.