Study On MiRNA-200b-3p In Insulin Resistance Of PCOS And The Impact Of TSH On Blood Glucose And Lipids Of PCOS

Part IStudy on miRNA-200b-3p in insulin resistance of PCOSBackground:Polycystic Ovary Syndrome(PCOS)is a common endocrine metabolic disorder Syndrome.Its major characteristics are oligo-/anovulation,hyperandrogenism and polycystic ovaries change.Clinical manifestations include menstrual disorder,acne,hair loss.It usually accompanied by insulin resistance(IR)and other multiple metabolic disorders,seriously affecting the physical and mental health of patients with PCOS.It is considered that IR is important in the development and progression of PCOS.Micrornas(miRNA)is the length of 21-23 nt small non-coding RNA that regular gene expression post-transcriptionally through cleavage or repression of the target messenger RNA(mRNA).MiR-200s is closely related to insulin resistance and diabetes,and a member of this family mir-200b-3p was highly expressed in granulosa cells of PCOS with IR patients.Objective:To explore the possible pathogenesis of miR-200b-3p in granulosa cells of PCOS with insulin resistance.Methods:Analysis of existing data,we screened the potential target genes of miR-200b-3p through bioinformatics and literatures analysis and explored the possible mechanism of miR-200b-3p affecting insulin signaling pathway.Results:MiR-200b-3p was high expressed in granulosa cells of PCOS with insulin resistance patients when comparing with the patients without IR and the normal(4.71 ± 1.41 vs 1.07±0.11,P<0.05;4.71 ± 1.41 vs 1.12±0.10,P<0.001),and miR-200b-3p was positively associated with HOMA-IR(r=0.532,P<0.01).After analysis,we chose the target gene PTEN.After transfection and luciferase experiment,it proved that miR-200b-3p can combine with the 3 ’UTR region of PTEN.Conclusion:MiR-200b-3p may participate in insulin signaling pathway through binding PTEN.Part IIThe impact of TSH on blood glucose and lipids of PCOSBackground:Polycystic Ovary Syndrome(PCOS)is a common endocrine metabolic disorder Syndrome.Its major characteristics are oligo-/anovulation,hyperandrogenism and polycystic ovaries change.Clinical manifestations include menstrual disorder,acne,hair loss and acanthosis nigricans.It usually accompanied by insulin resistance and other multiple metabolic disorders and long-term complications such as cardiovascular and cerebrovascular accident,endometrial cancer,seriously affecting the physical and mental health of patients with PCOS.Studies have shown that the prevalence rate of autoimmune thyroiditis and the thyroid stimulating hormone(TSH)levels are increasing in PCOS patients.The clinical characteristics and metabolic indicators between them are widely concerned,but the relationship is still unclear.Objective:To analyze the characteristics of TSH and its effect on blood glucose,lipids in PCOS patients.Method:1034 PCOS patients without a recent history of thyroid disease treatment were collected to measure height,weight,waist circumference(W C),hip circumference(HC),blood pressure,thyroid stimulating hormone(TSH),sex hormones,blood glucose,insulin,serum lipids,and calculated the body mass index(BMI),waist hip ratio,LH/FSH,HOMA-IR.Based on our hospital normal TSH cut-off,PCOS patients were divided into two groups,normal TSH group(0.27 uIU/ml≤TSH≤4.2 uIU/ml)and higher TSH group(TSH>4.2 uIU/ml)and compared their biochemical indicators.Results:The mean age of the 1034 women was 29.47±3.75 years and their BMI was 25.41±4.68kg/m2.163(15.76%)had higher TSH level.Body weight,BMI,WC,systolic pressure,diastolic pressure were statistically significant.The proportion of overweight patients in higher TSH group was significantly higher.The two groups of 2h blood glucose,FINS,2h insulin and HOMA-IR were not statistically significant,but PCOS with higher TSH had a rising trend.Higher TSH group of TC(4.52±0.81mmol/L vs 4.64±0.81mmol/L,P=0.027),TG(1.48±1.18mmol/L vs 1.66± 1.26mmol/L,P=0.022),LDL-C(3.03±0.64mmol/L vs 3.13±0.6mmol/L,P=0.049)were higher than that of TSH normal group,and the differences were statistically significant.TSH showed a weak positive correlation with hip circumference,blood pressure,FINS,2h insulin,HOMA-IR,TC,TG and LDL-C(P<0.05).Conclusion:Dyslipidemia was increased in PCOS with TSH elevated.TSH screening should be measured in PCOS patients.