Preparation Of Electrochemical Luminescence-enhanced Nanoparticles Based On Dopping Ionic Polymers And Its Immunoassays

In this paper,the preparation of electrochemical luminescence-enhanced nanoparticles based on dopping ionic polymers and its application in the quantitative detection of hepatitis B surface antigen(HBsAg)were studied.Based on the coordination reaction of ruthenium trichloride hydrate with 2,2’-bipyridine to give ruthenium bipyridine,the synthesized bipyridyl ruthenium and ligand with epoxy ring group 5,6-dioxo-[1,10] phenanthroline was coordinated to obtain an electrochemical luminescent ruthenium complex Ru-1.The ruthenium complex Ru-1 was attached to the PAA chain using an epoxy ring-opening reaction between the carboxyl group above the polymer macromolecμle polyacrylic acid(PAA)and the epoxy group on the synthetic ruthenium complex Ru-1.The electroluminescent polymer macromolecules PAA-Ru-1 with different grafting rates were prepared.Based on the study of UV,fluorescence spectra,ECL and grafting rate,PAA-Ru-1 with higher grafting rate was prepared to prepare ECL nanoparticles based on hydrophobic interaction.The crosslinked nanocomposites([Ru-PAA/PAA]-NPs)were prepared by crosslinking and crosslinking the non-luminescent polymer PAA while crosslinking the PAA-Ru-1 with aziridine as the crosslinking agent.However,the prepared luminescent intensity of crosslinked nanoparticles is not very good.On the basis of this,the ECL nanoparticles were prepared by hydrophobic interaction of PAA-Ru-1.The effects of PAA and PEI on the strength of ECL were investigated.The structure was stable,Strong ECL,water-soluble nanoparticles.A highly sensitive electrochemiluminescence of hepatitis B surface antigen detection system was constructed.The linear range of this method is 1.0-100 pg/ml,the detection limit was 0.049 pg/ml(S/N = 3).Compared with other HBsAg detection methods,this method has the advantages of high sensitivity and good reproducibility.At the same time,the system does not have significant interference in the simμlated plasma sample analysis,and is expected to be applied to the detection of clinical real samples.