Experimental Study On The Repair Of Articular Cartilage Defect Of Knee Joint In SD Rats By Synovial Mesenchymal Stem Cells And Chondrocytes

Objective:Cartilage tissue damage is the main feature of Temporomandibular joint os teoarthritis,so the most necessary problem to solve in present clinical work is the hard hea lness after the cartilage damage.We investigate the chondrogenic differentiation of synovial mesenchymal stem cells co-cultured with chondrocytes on the chitosan/type I collagen composite scaffolds after being transplanted into the Sprague-Dawley rats‘ Knee joint defect model.Methods: The synovial membrane and articular cartilage were harvested from rat knee joint.The synovial mesenchymal stem cells and chondrocytes harvested from the synovial membrane and articular cartilage of Sprague-Dawley rats were obtained by enzyme digestion method and cultured respectively.Passage 3 synovial mesenchymal stem cells and passage 2 chondrocytes,which were divided into four groups: group A(chondrocytes alone),group B(synovial mesenchymal stem cells alone),group C(ratio of synovial mesenchymal stem cells:chondrocytes=1:2),group D(scaffold material without cells),and group E(sham operation).Group a、Group b、Group c、Group d were cultured on chitosan/type I collagen composite scaffolds and transplanted into the Knee joint defect followed by morphological observation and immunohistochemical staining at4 、 8 、 12 weeks.Results:After 4,8,12 weeks,The immunohistochemical staining of type II collagen and the toluidine blue staining of aggrecan were significantly positive in groups A and C.The co mbination of two kinds of cells cultured in chitosan / type II collagen composite scaffold can accelerate the healing of cartilage defects and improve the quality of cartilage repair.Conclusion:Its acquisition is simple,the clinical feasibility is strong,at present,the re is a broad prospect in clinical application.