The Effects Of Folate Levels On Endometrial Receptivity And The Level Of HOXA10 Gene Methylation Were Studied

ObjectiveEndometriosis(EMs)is a kind of common disease related to female infertility,HOXAIO gene is one of the susceptible genes of endometriosis,Folic acid is a group of B vitamins that bear a carbon unit during the growth and development of mammals.It is an essential vitamin for the synthesis of human DNA.Folic acid,as the ultimate source of methyl group in human body,plays an important role in maintaining DNA methylation.The purpose of this study was to investigate the relationship between endometrial receptivity of plasma folate and DNMTs gene,HOXA-10 gene and EMs infertility patients,in order to provide a new treatment for infertility patients to improve the probability of pregnancy in EMs.Methods2016-09 to 2017-09 at Changzhou maternal and Child Health Hospital Reproductive Center for infertility patients of IVF 30 were analysed,all subjects underwent laparoscopic operation with clear pathological diagnosis。The causes of infertility were 15 cases of endometriosis(AFS stages:Ⅲ-Ⅳ period),and 15 of tubal factors were the control group(Bilateral tubal ligation or ostomy).The levels of plasma folate and CA125 in two groups were detected by ELISA kit,and two groups of patients were monitored by B ultrasound.The endometrium were taken after seven days of ovulation,and the expression of HOXA10 RNA m was detected by fluorescent quantitative PCR.The expression of HOXA10 in endometrium was detected by BSP.Using SPSS 22.0 statistical software processing,the general data of patients were analyzed by single factor analysis.Comparison of counting data with chi 2 test;The measurement data of the two or two comparisons were first conducted in the normal test and the variance homogeneity test,and the variance was tested by t-test,whereas the approximate t test was used.The variance analysis was used to compare the normal distribution with the normal distribution,and the difference between P<0.05 was statistically significant.Results(1)Results showed that the folic acid content in normal group were significantly higher than that of folic acid in plasma of patients with endometriosis,there was no significant difference between the two groups of patients with CA125;(2)The results showed that the expression level of HOXA10 gene in M RNA.was significantly higher than that in endometriosis group in the normal group,the expression level of DNMT1,DNMT3a,DNMT3b was significantly lower than that in endometriosis group in normal group;(3)The results showed that patients with endometriosis DNA methylation and normal group were compared,there were significant differences in the average level of CpG methylation,often in endometriosis group(82%)was significantly higher than the normal group(57%group),two compared with significant differences;(4)The results of correlation analysis showed a significant correlation of methylation level and endometrial tissue of patients with folic acid,folic acid content in the body is low,the higher the degree of methylation in patients with endometrial.ConclusionThe expression of HOXA10 gene in patients with endometriosis is low,but the probability of methylation is significantly higher tharn the control group,indicating the presence of HOXA10 gene methylation of DNA in endometriosis patients,the aberrant methylation may be the cause or the patient is sick and the receptivity of endometrium down one of the important reasons.The folate level in EMs group was significantly positive correlation with HOXA10 gene,and significantly lower than normal group.By increasing endometrial tolerance and reducing the promoter methylation rate,the EMs progress or license can be prevented as a new way to treat EMs.