Study On The In Vitro Amplification Ability And Clinical Analysis Of CIK Cells In Cancer Patients

Biotherapy serving as the fourth treatment means is increasingly focused,and has been clinically applied.Adoptive immunotherapy is an important branch of the biotherapy and is characterized by culturing and amplifying the leukomonocytes with anti-tumor performance in the tumor patients with interleukin-2(IL-2)in vitro until certain number,and then back-infusing to the body of the patient to kill the tumor cells with the leukomonocytes with anti-tumor performance to achieve the anti-tumor purpose.CIK cells are the main force of the adoptive immunotherapy,and compared with other anti-tumor medicines,can directly kill tumor cells on the premise of mot damaging the organism immune system structure and functions,and can adjust and enhance the organism immune functions.Clinically,the mature CIK cells are mainly obtained by collecting mononuclearcells in the peripheral blood of the tumor patients and carrying out in-vitro induction and amplification,and then used for cell adoptive therapy.In this article,by comparing different culture methods,such as,changing culture medium,culture conditions,culture containers,and cultured cell factors,to research the in-vitro amplification capability of the CIK cells of the tumor patients.The experiment result shows that by adopting a G-Rex culture tank to culture the CIK cells,the cell proliferation capability is about 90 times,and the expression of the CIK phenotype can be significantly improved;the survival rate of the CIK cells is improved to a certain extent;GT-T551 is not suitable for culturing the CIK cells for long time;the proliferation effect of the AIM-V culture medium is higher than that of the VIVO culture medium,the mature time of the cells cultured by the AIM-V culture medium and the VIVO culture medium is different,and the cells in the best state cultured by the AIM-V culture medium and the VIVO culture medium are not obvious in the difference;X-VIVO is also used for culturing the CIK cells in the absence of FBS,but the cell proliferation is obviously reduced and the cell phenotype is also poor in comparison with those in the presence of FBS;addition of the IL-1α in the CIK cell culture is capable of improving the expression amount of CD28 by about 10%.In this article,by comparison and research on the in-vitro killing activity to the tumor patients of different pathogeny,the CIK cells of the tumor patients are verified to have killing action on partial tumors.