Effect Of Astragalus Polysaccharides On Myeloid Derived Suppressor Cells In Patients With Non-small Cell Lung Cancer

Objective:To investigate the effect of co-culture of astragalus polysaccharides(Aps)and myeloid-derived suppressor cells(MDSCs)in the peripheral blood of non-small cell lung cancer patients in vitro,to observe the effect of Aps on MDSCs subsets,and to further explore the anti-tumor immune mechanism of Aps,providing a new basis for the clinical astragalus polysaccharide immune adjuvant therapy.Methods:Circulating peripheral blood and cancer tissue from 25 patients with non-small cell lung cancer(NSCLC)and the circulating peripheral blood of 23 healthy patients of control group were diagnosed using histopathological or cellular diagnosis.Percentages of MDSCs(HLA-DR-CD14-CD33~+cells)in peripheral blood mononuclear cells(PBMNCs)and tumor tissue single cell suspension were measured by flow cytometry.PBMNCs from patient with NSCLC were co-cultured with astragalus polysaccharides,and percentages of MDSCs were also measured by flow cytometry.Then analyzing MDSCs expression change before and after Aps treatment.Results:1.Percentages of HLA-DR-CD14-CD33~+MDSCs in peripheral blood from 25 cases with NSCLC patients before surgery(1.65±0.52)%was significantly higher than that of healthy subjects(0.33±0.13)%(p<0.01).2.Percentage of HLA-DR-CD14 CD33~+MDSCs in tumor from patients(0.98±0.33)%was significantly higher than that MDSCs in peripheral blood of healthy subjects(0.33±0.13)%(p<0.01).3.HLA-DR-CD14-CD33＋MDSCs from the same patient in tumor tissue(0.33±0.13)%were significantly lower than that in peripheral blood(1.65±0.52)%(p<0.01).4.MDSCs in peripheral blood was reduced significantly after co-cultured with astragalus polysaccharides(p<0.01).Conclusions:HLA-DR-CD14-CD33~+MDSCs is involved in the progression of non-small cell lung cancer diseases,regulating the immune function of the patients;The percentage of HLA-DR-CD14-CD33~+MDSCs in peripheral blood of non-small cell lung cancer decreased after co-culture with astragalus polysaccharide,Aps may regulate the immune function of patients through promoting the differentiation and maturation of MDSCs.