Effects Of Exosomes Derived From Mutant HIF-1a Modified Bone Mesenchymal Stem Cells On Chondrocyte Apoptosis And Its Role On The Regeneration Of Early Cartilage Defects Of The Rabbits Combined With Porous Scaffold

ObjectiveCartilage defects is one of the common joint diseases throughout the world.We hypothesized that exosomes may have supporting role in treating this disease.MethodsIn vitro,bone marrow mesenchymal stem cells(BMSCs)were transfected with mutant HIF-1α.Exosomes derived from both kinds of BMSCs(BMSC-Exonor or BMSC-Exomu)were isolated and identified.Chondrocytes were handled with TNF-α,and BMSC-Exonor or BMSC-Exomu were also added at the same time.Proliferation,apoptosis,and the activity of MAPKs and Akt were examined.In vivo,BMSC-Exonor or BMSC-Exomu were combined with porous scaffold to repair cartilage defects,and the efficacy appraisal by histological examination.ResultsWe found that BMSC-Exomu could dose-dependently promote the proliferation of chondrocytes,and inhibit apoptosis induced by TNF-α.And the effects were much stronger compared with BMSC-Exonor,and were partially dependent on MAPKs and Akt signaling pathways.While in vivo,BMSC-Exomu improved regeneration of early cartilage defects of the rabbits combined with scaffold.ConclusionsThese findings highlight the supporting potential of BMSC-Exomu in repairing cartilage defects.Such exosomes combined with porous scaffold may represent a novel approach for the treatment of cartilage defects.