Screening Of Specific Genes For Liver Cancer

Background:Liver cancer is the fifth most common cancer in the world and the third in mortality.China is a country with high incidence of liver cancer.Nearly 300 thousand people die of liver cancer every year,and the mortality rate is second,second only to lung cancer.Therefore,exploring the pathogenesis of liver cancer is extremely important.It has been proved that both hepatitis C virus and hepatitis B virus are the main causes of liver cancer in the world today.The viruses cause liver cancer because massive inflammation,fibrosis and eventual cirrhosis occurs within the liver.Many genetic and epigenetic changes are formed in liver cells during HCV and HBV infection,which is a major factor in the production of the liver tumors.Therefore,we speculate that the immune level of the patients with liver cancer has also changed.In addition,in recent years,a large number of experimental groups have sequenced whole genome or exon group of liver cancer,which has improved the gene mutation map of liver cancer,and is helpful for further understanding the pathogenesis of liver cancer.Objective: To explore the expression changes of related lymphocytes in hepatocellular carcinoma patients,and to screen specific genes that play a role in the occurrence and development of hepatocellular carcinoma through high-throughput sequencing technology.Method:1 PBMC was isolated from the peripheral blood of the liver cancer patients and normal healthy people,and the changes in the immune level of the patients were detected by flow cytometry.2 The specific genes were screened by high flux sequencing by extracting the RNA from the patient’s cancer and paracancerous tissues and PBMC of the patients and the healthy people.3 The specific genes were tested by RT-PCR,Real time-PCR and other experimental techniques.Results: Compared with normal healthy people,analysing the PBMC in liver cancer by FCM,its find that the expression of CD3 and CD4 decreased,CD8 expression increased,CD4/CD8 decreased,and the immune regulation of patients was abnormal.The detection of these cells can be used to diagnose the occurrence of liver cancer and the dynamic observation of the therapeutic effect.High throughput sequencing technology screened specific genes XIST,FOXP1,HAGLR,DIO3 OS and MEG3,Real-time PCR identified the expression of specific genes FOXP1-AS1,HAGLR and MEG3 in liver cancer,suggesting that they are involved in regulating the occurrence and development of liver cancer,so as to provide new ideas and new targets for gene therapy of liver cancer.Conclusion: Compared with healthy people,the liver cancer patients have abnormal immune regulation,which can be used as diagnostic markers.At the same time,the abnormal expression of FOXP1,HAGLR and MEG3 in the patients with liver cancer can provide help for the research of gene therapy.