The Study Of The Influence Of Angiotensin ?/ AT1R Inhibitor On The Growth And VEGF MRNA Expression Of The HepG2 Transplanted Subcutaneously In Nude Mice

Objective: To study the effect of human angiotensin ?(Ang?)and Angiotensin ? receptor antagonist inhibitor on growth the expression of vascular endothelial growth factor(VEGF)m RNA in tumor tissue of the Hep G2 nude mice model with xenograft of human hepatocellular carcinoma.Methods: Human hepatocellular carcinoma Hep G2 cells were resuscitated and cultured to logarithmic growth phase,and the cell suspension concentration in logarithmic growth phase was adjusted to 3 × 107 cells / ml.After the nude mice were disinfected,the cell suspension was inoculated subcutaneously in the back of the nude mice,each inoculated with 0.2ml.The tumor nodule diameter is more than 5mm,which is regarded as a successful model,and it is generally 10-12 days in nude mice.The nude mice were randomly divided into three groups: control group,losartan feeding group,Ang? intraperitoneal administration group,10 rats in each group.The In addition to the normal diet,the control group of nude mice given half of 0.9% sodium chloride 0.5ml gavage,the other half given 0.9% sodium chloride 0.1ml intraperitoneal administration;losartan feeding group daily given losartan solution(30mg/ Kg / d)0.5ml gavage;Ang? group given 50 ug / kg nude mice intraperitoneal administration 0.1ml.Three groups of nude mice were housed in SPF feeding room,and the tumor growth status was observed daily.The changes of tumor size were recorded.After 21 days of continuous administration,the rats were sacrificed for 2 days,and all nude mice were sacrificed.The tumor tissue was carefully removed and the size was measured and weighed.The maximal diameter(a),the shortest diameter(b),and the tumor volume V = 1/2 ab2 were measured.The expression of VEGF m RNA in tumor tissue was detected by real-time PCR,and the Ct values of the tested gene and internal reference gene were read.The relative expression of the gene to be detected was calculated by the formula R = 2(CT internal reference-CT gene).Results: The expression of VEGF m RNA in tumor tissue of nude mice models was enhanced by the Ang? which was used by the mean of intraperitoneal injection.And the VEGF m RNA expression of tumor tissue of nude mice models which were given intragastric administration with Losartan was obviously inhibited.There are significantly statistical differences between these two groups in the expression of VEGF m RNA in tumor tissue of nude mice models(P<0.01).And there are also significantly statistical differences between the control group and the administration with Ang? group(P<0.01).Identically,statistical differences between the control group and the administration with Losartan could also be discovered(P<0.01).Conclusion: Angiotensin ?(Ang?)could enhance the expression of VEGF m RNA in tumor tissue of nude mice models and also could speed up the growth of tumor tissue of the Hep G2 nude mice model with xenograft of human hepatocellular carcinoma.On the contrary,Angiotensin ? receptor(AT1R)antagonist such as Losartan could inhibit the expression of VEGF m RNA in tumor tissue of nude mice models.