Study On Mesoporous Calcium Silicate Doping Calcium Sulfate Bone Cement For Repair Defects Of Bone Trauma

The calcium sulfate bone cement(CSC)is the most recent focus of a new type of bone repair materials.CSC has good plasticity and biocompatibility,CSC can be solidified in physiological conditions.However,with the deepening of the clinical application and research,found the degradation speed of regular CSC is too fast,difficult to reach the effect of support at the site of the defect.Therefore,modification was carried out on the CSC to slow its degradation performance and promote osteogenesis effect has become the focus of research.The Study prepared mesoporous calcium silicate(m-CS)by sol-gel method,research the physical and chemical properties by XRD,IR,TEM and SEM.A novel bone cement of m-CS/CSC(m-CSC)was fabricated by introducing mesoporous calcium silicate(m-CS)into calcium sulfate bone cement(CSC),and physical-chemical and biological performances(including repair defects of bone trauma)of m-CSC were studied both in vivo and in vitro.The setting time and compressive strength of the m-CSC(CSC used as control)were tested.The cement samples were immersed in Tris-HCl solution,and the in vitro degradation of the m-CSC was measured.The morphology of MC3T3-E1 cells on the cements was observed using confocal laser scanning microscopy(CLSM),and the proliferation and differentiation of MC3T3-E1 cells cultured on samples were assessed using CCK-8 and ALP kit,respectively.The cements were implanted into the bone defects of femur bone trauma of rabbits,and the in vivo degradability and osteogenesis of the cements was investigated by synchrotron radiation X-ray,histological elevation and immunohistochemical after implantation for 4,8 and 12 weeks.The addition of m-CS into CSC prolonged the setting time and promoted the degradability of the m-CSC,which did not have obvious effects on the compressive strength of the cements.In cell culture experiments,the result showed that the m-CSC could promote the proliferation and differentiation of C3T3-E1 cells.The bone defects were repaired after m-CSC cements were implanted for12 weeks.The results of histological elevation and immunohistochemical staining from animal model revealed that massive bony tissue formed while the cement obvious degraded,indicating that the osteogenesis of the m-CSC were better than CSC,and the positive express ratio of VEGF and COLL I of the m-CSC were also better than CSC.The results showed that m-CSC had good biocompatibility and osteogenesis,which could promote bone regeneration and repair bone trauma.