Coronary Heart Disease Epicardial Fat Lipid Study On Metabolic Mechanism

Objective: Lipid metabolism of fat cells has an important effect on the formation of coronary heart disease.The purpose of this study:First,the differences in lipid metabolism in patients with coronary heart disease and non-coronary heart disease were preliminarily studied.Second,the mechanism of changes in lipid metabolism of fat cells in high fat,sugar and inflammatory states was simulated in vitro.Methods:First,the collection of epicardial adipose tissue.Epicardial fat was divided into coronary heart disease(20 cases)and non-coronary heart disease group(20cases).First,the content of glycerol and triglycerides in adipose tissue was determined by enzymatic method.Followed by Western blot detection of adipose tissue perilipin A,ATGL and HSL.Finally,the concentration of c AMP and PKA in EAT was detected by ELISA.In vitro culture of 3T3L1 induced differentiation into mature adipocytes,respectively,to give different concentrations of ox LDL,LPS,glucose and continue to culture for 2 days.1)Staining of lipid droplets with Bodipy 493/503.Determination of triglyceride and glycerol in cells by enzymatic method.The contents of perilipin A,ATGL and HSL in the cells were detected by Western blot.The concentration of c AMP and PKA in the cells was detected by ELISA.3D microscopy was performed using IPP6.0 to calculate the percentage of lipid droplets in cells to calculate the percentage of small droplets and large fat droplets.Results: Compared with the epicardial adipose tissue of non-coronary heart disease group,glycerol and triglyceride levels were decreased(P <0.05),perilipin A increased(P <0.05)、ATGL and HSL were decreased(P <0.05),PKA,c AMP content decreased(P <0.05).1)Compared with low and medium ox LDL,the concentration of small droplets in 3T3L1 cells treated with high concentration was decreased and the lipid droplets increased(P <0.05),glycerol content decreased(P <0.05),triglyceride content increased(P <0.05),perilipin A increase,the levels of ATGL,HSL,PKA and c AMP were decreased(P <0.05).2)Compared with glucose-free and low-concentration glucose,the concentration of small droplets in 3T3L1 adipocytes treated with high concentration of glucose was decreased and the lipid droplets increased(P<0.05),glycerol content decreased(P <0.05),triglyceride content increased(P<0.05),perilipin A increase,the levels of ATGL,HSL,PKA and c AMP were decreased(P <0.05).Low concentrations of glucose and glucose-free also showed the same results.3)different concentrations of LPS compared to high concentrations of LPS treatment of 3T3L1 cells can be obtained in small droplets increased,large fat drop,glycerol content increased(P <0.05),triglyceride content decreased(P <0.05),However,there was no statistically significant difference in the levels of perilipin A,ATGL,HSL,c AMP and PKA.Conclusion:Compared with the non-coronary group,the epileptic fat cells of the coronary heart disease group were inhibited.So coronary heart group epicardial fat cell accumulation.High concentrations of ox LDL,high concentrations of glucose inhibit 3T3L1 cell lipolysis,by regulating c AMP-PKA pathway,perilipin A-ATGL-HSL to inhibit 3T3L1 fat cell lipolysis.High concentration of LPS can promote the hydrolysis of 3T3L1 adipocytes,mainly due to increased fat droplets,large drops of fat drops,glycerol content increased,triglyceride content decreased.However,LPS did not regulate the lipolysis of 3T3L1 adipocytes by c AMP-PKA and perilipin A-ATGL-HSL pathway.