Effects Of Different Training On Changes Of Active Oxygen Threshold And Lactate Threshold In Skeletal Muscle Of Rats

In the incremental load to exhaustive exercise,the body of reactive oxygen species?ROS?and the blood lactate?LD?has a dynamic change rule like a sudden increase,and the concentration change is closely related to the exercise load.ROS sudden increase?threshold?represents the imbalance of the ROS generation and anti-oxidation clearance.It has important application value for oxidative damage monitoring.In this paper,we compared the training group and the training group to complete the same conditional incremental load exercise,to verify the objective existence of ROS threshold,and to observe whether there was a decrease in the ROS threshold in the blood and skeletal muscle after completion of the same conditional incremental exercise compared with the training group and the untreated group.At the same time,the dynamic changes of LD and creatine kinase?CK?in blood were observed,to provide experimental evidence for further study of the mechanism.Methods:42 male Wistar rats of 6 weeks old were randomly divided into two groups: no exercise group?E6 group,6 subgroups,3 subgroups each,a total of 18?,aerobic exercise group?E8 group,and then divided into eight subgroups,3 subgroups each,a total of 24,for 3 weeks swimming training?.After 24 hours of swimming at the end of the E8 swimming group,the rats in the two groups were subjected to the same condition of one-time incremental load exhaustive swimming.The first load began from no weight,and then each load increased 1% of body weight,each level of load swimming for 5min.The rats in each subgroup were anesthetized immediately after the corresponding load was completed,then taken blood,soleus muscle,red gastrocnemius and white gastrocnemius.The contents of malondialdehyde?MDA?,H₂O₂,LD and the activity of CK,SOD in blood and muscle were detected by the kit.Results:1.The two groups of rats in the same conditions to complete the incremental load movement,the changes of the content of MDA,H₂O₂,LD and the activity of CK,SOD in blood.?1?The contents of MDA,H₂O₂ and the activity of CK in the blood were significantly different from those in the fifth grade?P <0.01?in the E6 group and the load of the sixth grade in the E8 group?P <0.05?.?2?The contents of LD in blood E6 group 5 level load and the fourth level of load compared to a very significant difference?P <0.01?,There was a significant difference between the 6th grade load and the fifth grade load in the E8 group?P <0.05?.?3?There was no significant difference in the activity of SOD in the blood between the E6 group and the E8 group?P> 0.05?.2.The two groups of rats in the same conditions to complete the incremental load movement,the changes of the content of MDA,H₂O₂ and the activity of SOD in the soleus muscle,red gastrocnemius muscle and white gastrocnemius muscle:?1?The content of MDA: E6 group in the soleus muscle,red gastrocnemius,white gastrocnemius 6 load compared with the level 5 load There were significant differences?P <0.01,P <0.01,P <0.05?.;In the E8 group,the content of red gastrocnemius muscle and white gastrocnemius muscle was significantly different from that of the seventh grade load?P <0.01?,and the difference between the 7th grade and the 6th grade of the soleus muscle was significantly different?P <0.05?.?2?The activity of SOD: E6 group and E8 group of three muscle types of each load and the previous level of load were no significant difference?P> 0.05?.?3?The content of H₂O₂: E6 group in the soleus muscle,red gastrocnemius 6 load compared with the fifth level load?P <0.01?.In the E8 group,the imalus muscle grade 7 load was significantly higher than that in the fourth stage?P <0.05?,and there was significant difference between the fifth dose of the gastrocnemius muscle and the fourth grade load?P <0.01?.There was significant difference between the fifth stage load of the red gastrocnemius and the gastrocnemius muscle?P <0.01,P <0.05?.Conclusion:1.The ROS thresholds in blood and muscle tissue of E6 group and E8 group were all objective,and the ROS threshold in blood was lagging behind the appearance of blood lactate threshold when the same conditional incremental exercise was completed.2.The ROS threshold in blood and muscle of E8 group was delayed in E6 group.This may be due to aerobic training that enhances mitochondria in oxidative phosphorylation of aerobic energy levels and improves the ability of antioxidant.3.The increase in CK activity in the blood occurs after the blood lactate threshold,consistent with the ROS threshold in the blood.Indirect description of the ROS threshold corresponding to the exercise load may be exacerbated by the critical load of oxidative damage,resulting in CK from intracellular flow into the blood of the vital capacity was significantly increased.