| Nervonic acid(C24:1, ? 15) is an important element of the nerve cells and nerve tissue. It can promote the repair and regeneration of the damaged nerve cells and tissue. In recent years, the source of nervonic acid is changed into aquatic algaefrom shark oil. At this stage, the main research is to improve the production efficiency. But the study of adding external reagent to increase nervonic acid production is less.Microalgae Mychonastes afer was screened in 2011, which has high nervonic acid content. The content of novernic acid can get accounts for 5% of total oil from M.afer. This discovery has important economic value. In the process of microalgae cultivation, the plant growth regulators and antioxidants as additive can improve product yield. Plant growth regulator is also known as plant hormones. It is widely used in growth and development of crop. Some of the plant growth regulator can improve the contentof protein, sugar and fatty acid. Antioxidants play a role in biological body by electron transfer, complexation metal ions, combined antioxidant effect and affect gene expression, etc. Antioxidants are widely used in food, medicine, health products and cosmetics industries. In particular, the antioxidants can effectively inhibiting lipid oxidation in biological accumulation stage.In our experiment, we use the 6 additives. Naphthalene acetic acid(NAA), brassinolide(BR), benzyl amino purine(BAP) are used as the plant growth regulator. Butyl hydroxylanisole(BHA), epigallocatechin gallate(EGCG) are used as the antioxidants. After study, we found that NAA can effectively improve nervonic acid production. In 4d, the ratio ofnervonic acid to total fatty acid is 6.344% from the group which was added 0.3mg/L NAA. It was 73% higher than the control group and the orthers experimental groups. In addition, 1?M/L EGCG was added to the medium. In 2d, the ratio of total oil weight to dry weight was 61.1% in microalgae. The proportion of this group was 10% higher than the other groups. The groups of the other 3 kinds of additive didn't have any improvements in all the data.The study has shown that NAA and EGCG can improve the nervonic acid production of the microalgae M.afer. And the effect was obvious. Adding NAA can improve the biomass and nervonic acid content of microalgae?And EGCG can improve the fatty acid accumulation in the cells. According to these conclusions, thecollaborative experimentwas designed in the final stage. Both of NAA and EGCG were used in the experiment. The results of the experiment showed that the effect of the combination is better than a single use.Meanwhile, the experiment monitored chlorophyll fluorescence parameters. The effects of Two Additives on M.afer photosynthetic efficiency were analyzed. The results showed that, NAA had no influence on photosystem ?(PS?) of M.afer, it has activation on light system I(PSI). The data of integrated dual optical system showed that NAA can promote the photosynthesis of M.afer; EGCG improve photosystem ?(PS?) active and inhibit photosystem I(PSI) activity of M.afer. The data of integrated dual optical system showed no effect on the overall efficiency of photosynthesis in M.afer with EGCG. |
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