Study On Anaerobic Fermentation Of Electrocoholized Lignocellulose

Currently, a large area of rural aquaculture rises in Erhai Lake Basin, especially in the northern part of Lake Erhai basin, which caused huge non-point source pollution in rural, and threatening water quality in Erhai Lake Basin. Therefore, it is necessary to control the pollution, and cow manure handling is one of the important means to solve nonpoint source pollution. If cow manure is used to anaerobic fermentation, it not only reduces pollution and produces biogas in rural areas, but also be used to produces organic fertilizers using the anaerobic fermentation residues. But the cow manure contains lignocellulose complex, which is difficult to be degraded by microorganisms in the anaerobic fermentation process. What’s more, it is very negative for medium-sized and household biogas production, thus to find a way to improve the efficiency of lignocellulosic degradation method is particularly important. This paper explored the influence factors on anaerobic fermentation of enzymatic pretreatment, electrochemically assisted enzymatic pretreatment and electrochemical conditions. Meanwhile, the degradation of lignocelluloses in the process of cow manure anaerobic fermentation was analyzed, as follows:1.Electrically assisted enzymatic pretreatment experiments to determine optimum conditions for dairy manure by single factor:Solid-liquid ratio was 1:15, the temperature was 50 ℃, voltage was 0.8v, with the cathode electrode. Reducing sugar yield which was produced at Ov,0.8v anode and 0.8v cathode auxiliary conditions cellulase was 5.62%, 6.43% and 6.99% respectively. We found that enzymes can promote the secondary electrochemical generation of reducing sugars, and the effect of cathode auxiliary was better than anode. No voltage auxiliary cellulase, anode with cellulase, anode without cellulase, cathode with cellulase and cathode without cellulase were obtained reducing sugar yield of 5.62%,6.43%,6.11%,6.99%, and 6.6%. It is can improved that the electric auxiliary enzymes promote the formation of sugar.From the point of view of cellulose degradation rate, Ov,0.8v anode and 0.8v cathode cellulose degradation rate was 7.62%, 14.15% and 31.69% respectively. More telling, The effect of the cathode is better than the anode, electric auxiliary can promote the degradation of cellulose.2. Enzymatic pretreatment can promote the lignocellulose degradation and to improve the quality of biogas by anaerobic fermentation effectively. Raw materials directly anaerobic fermentation and enzymatic pretreatment anaerobic fermentation methane content and total gas production were about 60%,9705.3mL and about 65%,11272mL. The anaerobic fermentation of Enzymatic pretreatment methane content and total gas production were higher than the anaerobic fermentation of raw materials. It showed enzymatic pretreatment can promote cow manure in lignocellulose degradation, improve the quality and quantity of biogas production by anaerobic fermentation biogas.3. Under cathodic conditions,0.3v,0.8v,1.5v and 2.5v assisted total gas production were 11763mL,10592mL,10436mL and 9106mL respectively. Methane content in different cathode voltage differed is largely, but were higher than the amount of methane produced by direct fermentation. Maximum amount of methane was produced under 0.3v conditions but the content of volatile methane conditions under 0.8v voltage conditions fluctuated relatively smaller than in the other three to the production of methane content, the effect rate of degradation of lignocellulose was the most obvious.4.The gas production of fermentation process, enzymatic pretreatment anaerobic fermentation 0.8v cathode (11321mL)> enzymatic pretreatment anaerobic fermentation (11272mL)> anaerobic fermentation plus 0.8v cathode (10592mL). From the point of view of cellulose degradation rate,enzymes pretreated 0.8v cathode (59.02%)> enzymatic pretreatment fermentation 41.21%> Direct fermentation feedstock plus 0.8v cathode (41.05%), The pretreat effect of enzymatic and electrochemical interaction was obvious than individual action or enzymatic pretreatment effect.5.Form the known mechanism electrically assisted anaerobic fermentation, the electroch emical conditions can promote enzyme activity and improve lignocellulose degradation.