Preparation Of PES/HA Pore Structure Material And Its Cell Activity

In this study,PES/HA composites were formed by adding different mass fraction of hydroxyapatite(HA)in polyether sulfone(PES),sodium chloride,and ammonium chloride were used as pore forming agents to prepare novel three-dimensional porous composite materials.By measuring the water absorption,porosity and mechanical properties of porous materials,observing the porous material pore structure,and finally compare with the effects of particle size and content on the porous materials.Osteoblast MG-63 and myeloma U2 OS were selected to study the cytotoxicity and mechanism of PES/HA composites,and biocompatibility of the composites also can be evaluated.The preparation of porous materials with sodium chloride,and ammonium chloride as pore-forming agents found that: with the two kinds of pore-forming agent,and be prepared between the pores of the three-dimensional porous material,the water absorption of PES/HA composite porous material has no linear relationship with pore size;the water absorption of porous materials is related to the content of pore-forming agent and particle size;the pore size is related to particle size.The pore structure was characterized by optical microscopy and scanning electron microscopy.The pore structure of the porous material was prepared with sodium chloride,and the pore former is mostly square.The pore structure of the porous material prepared by ammonium chloride as the pore former is mostly irregular.The content of pore forming agent increases,the elongation at break decreases,the tensile strength decreases and the elastic modulus decreases.The thermal decomposition kinetics of PES/HA composites is showing that: the thermal decomposition reaction of the composites is 1,the reaction series does not change with the HA content and pore structure.With the increase in HA content: the activation energy of the composites increases,the thermal stability increases,and the pore structure has no effect on the activation energy and thermal stability of the composites.The mechanical performance shows that: with the increase of HA content,the elongation at break of the composites decreases,the tensile strength decreases and the elastic modulus increases.Morphological studies show that: compared with the control group,the cell morphology of HA leaching solution was normal and the number changed little.PES leaching solution in the normal morphology of cells,no significant necrosis of cells,the number decreased;With the increase of HA content,the density of the cells increased gradually;Electron microscopic observation of cell growth in PES/HA porous composites found: a large number of cells by extending a number of pseudopods attached to the surface of the hole material and the hole wall and the material firmly combined,cells in the material surface and the hole surface of the hole completely extended,cell metabolism is strong,the material is covered by cell secretions,the cell boundary is blurred,and some cells can be seen covering the stent hole.The results of adhesion experiments show that the cell adhesion rate of the composites increases with the increase of HA content.Cell cycle studies showed that PES had a blocking effect on cell cycle.As the HA content increases,the composite material accelerates the cell cycle.Cell proliferation assay showed that HA had no inhibitory effect on cell proliferation;PES had a lower cell proliferation than HA;with the increase of HA content,the effect of composite materials on cell proliferation increased gradually.With the increase of the concentration of the protein(4~64 mg/m L),the cell proliferation gradually decreased.When the concentration was more than 16 mg/m L,the cell proliferation was inhibited by 32~64 mg/m L.The cytotoxicity levels of the materials are grade I,non-toxic to the cells,but the material has an effect on the relative proliferation rate of the cells.The relative proliferation rate of HA was smaller and had no inhibitory effect on proliferation;with the increase of HA content,the relative proliferation rate of the composites increased gradually.The oxidative stress was induced by oxidative stress.PES increased the level of oxidative stress;with the increase of HA content,the composite material decreased the oxidative stress level.These data demonstrate that the material through the induction of cell oxidative stress,changes in cell cycle and apoptosis,regulate cell proliferation,affecting cytotoxicity.When the cells in the ROS level has been high may cause cytochrome C release into the mitochondria,and cause apoptosis and inflammatory response,compared with the control group,the number of PES and PES/HA composites decreased,the mitochondrial fluorescence intensity decreased,and the mitochondrial granule clarity decreased;In the PES/HA composites,with the increase of HA content,the number of cells increased,the mitochondrial fluorescence intensity increased,and the mitochondrial granule clarity increased,which was consistent with the results of apoptosis test.Compared with the control group,the expression of COX4 gene in PES and PES/HA complex was gradually increased,in PES/HA composites,with the increase of HA content,the cell gene expression level was decreased and the cell biocompatibility increased.To compare the effect of PES/HA composite on osteoblast MG-63 and myeloma cells U2OS: compared with U2 OS cells,PES/HA composites are more favorable for the proliferation of MG-63 cells,resulting in low levels of oxidative stress.Comparison of PES,PEEK two groups of materials on the impact of two cells found: compared with PES,PEEK is more conducive to cell growth,cell cycle,lipid peroxidation decreased.Comparison of PEEK,SPEEK two groups of materials on the impact of two cells found: compared with PEEK,SPEEK inhibited the proliferation of U2 OS cells,which was not conducive to the adhesion of the cells to the surface of SPEEK,the cell cycle and the increase of lipid peroxidation;SPEEK did not significantly inhibit the proliferation of MG-63 cells,increased cell adhesion,shorter cell cycle and decreased lipid peroxidation.