Regulation And Application Of Nano-morphology Of Hydroxyapatite Porous Ceramics In Cuttlefish Bone

In recent years,porous hydroxyapatite ceramics have attracted attention due to its wide applications in many fields which resulted from its ideal chemical properties and physical structure.Cuttlefish bone is an ordered porous material with high porosity,pore connectivity,and it can be transferred into porous hydroxyapatite ceramics as a biological template.It is well known that hydroxyapatite with a nano-structure have larger specific surface area,better protein and ion adsorption ability,and therefore can enhance the performance of hydroxyapatite in many fields.Therefore this study is focus on adjusting the nanostructure of cuttlefish bone transformed porous hydroxyapatite ceramics,and explore the application of cuttlefish bone transformed porous hydroxyapatite ceramics with different nano-morpologies in bone tissue engineering and heavy metal adsorption.1.The hydrothermal method was used to transform the cuttlefish bone into hydroxyapatite porous ceramics.Through the control of its phosphorus concentration and ammonium concentration,cuttlefish bones transformed porous hydroxyapatite ceramics of different nano-morpologies were achieved : large-scale cluster-like structure cuttlefish bone-transformed porous hydroxyapatite ceramics have a cluster diameter of 3μm and consist of 64 nm nanosheets.The medium-sized cluster-like structure of cuttlefish transforms hydroxyapatite porous ceramics with a cluster diameter of 2.5 μm,consist of 18 nm nanosheets;small-sized clusters of cuttlefish bones transforming hydroxyapatite porous ceramics,with a cluster diameter of 1.9μm,consist of 16 nm nanosheets;cuttlefish bone transformed porous hydroxyapatite porous ceramics with small sized hexagonal-prisms-like structure,composed of nano-columns with a diameter of 100 nm and a length of 500 nm;cuttlefish bone transformed porous hydroxyapatite porous ceramics with medium sized hexagonal-prisms-like structure,composed of nano-columns with a diameter of 200 nm and a length of 5μm;cuttlefish bone transformed porous hydroxyapatite porous ceramics with hexagonal-prisms-like structure,composed of nano-columns with a diameter of 2μm and a length of 6μm.2.Osteoblasts were co-cultured with Cuttlefish bone transformed porous hydroxyapatite ceramics with different nano-morhology.As a result of the experiment, it was found that the Cuttlefish bone-transformed porous hydroxyapatite ceramic with a small-sized cluster morphology can better promote cell adhesion,proliferation,and ALP expression.Therefore,the cuttlefish bone-transformed hydroxyapatite with a small size cluster structure is expected to be used for bone tissue repair.3.Cuttlefish bone transformed porous hydroxyapatite ceramics with different nanostructure were used for the adsorption of lead ions.It was found that the cuttlefish bone with different surface nanostructure had different maximum adsorption capacity for lead ion adsorption,among which the small-sized cluster-like cuttlefish bone transformed porous hydroxyapatite ceramic has the largest lead adsorption capacity of 1451mg/g;the large-sized cluster-like cuttlefish bone transformed porous hydroxyapatite ceramic has a lead adsorption capacity of 954mg/g;the small-sized hexagonal-prisms-like cuttlefish bone transformed porous hydroxyapatite ceramic has a lead adsorption capacity of 754mg/g;the large-sized hexagonal-prisms-like cuttlefish bone transformed porous hydroxyapatite ceramic has a lead adsorption capacity of 613mg/g;the smooth cuttlefish bone transformed porous hydroxyapatite ceramic has a lead adsorption capacity of 609mg/g.Further investigation of the adsorption mechanism of cuttlefish bone transformed hydroxyapatite shows that it depends on the “dissolution-precipitation” approach.In combination with the porous structure of cuttlefish bone and the surface nanostructure of hydroxyapatite,a heavy metal adsorption filter with good adsorption capacity and separation ability can be obtained.In conclusion.different nanostructure can be achieved on the surface of cuttlefish bone transformed porous hydroxyapatite ceramics by controlling the transformation conditions.The nanostructure of the small-sized cluster-like structure can promote cell adhesion,proliferation and ALP expression,and is expected to be used for bone tissue repair.The nanostructure of the small size cluster structure is also more conducive to the adsorption of lead ions,combined with the porous structure of the cuttlefish bone and the nanostructure of the small-sized cluster structure,it is expected to be used for heavy metal adsorption.