| Cerebral ischemic stroke is one of the major diseases threatening people’s health, which is the cause of death ranked in the top three of mortality. China is the high morbidity of cerebral vascular disease country, there are about two million people suffer from stroke every year. According to the statistics, cerebral vascular disease is yet the top cause of death in China. A third of the stroke patients die, another third of the patients are mutilated, it not only brings the sufferings to the patients, but also brings burden to the patients’ family and the society. Therefore, it is the significant value that developing the few side effects, inexpensive and effective drugs for treating the cerebral ischemic stroke.DBZ is a new compound which was studied from some Fufang Danshen formulas, and was synthesized with Danshensu and borneol with technology of organic synthesis. According to the pharmacological actions of Danshensu and Borneol, we speculated that DBZ would has the protective effects on cerebral ischemic stroke. In the study, we studied the effects of DBZ on the middle cerebral artery occlusion model in rats from neurological symptoms, histopathology, biochemistry and Inflammatory cytokines.1Acute toxicity test of DBZObject:To study acute toxicity of DBZ.Methods:To inject DBZ into the tail vein of mices. The acute toxicity reaction of mices was observed in the successive14days.The death rate of mices were recorded, LD50was calculated by Bliss method.Results:The tail of mices died were necrosis, LD50was460.7mg/kg.Conclusion:DBZ had little acute toxicity.2Effect of DBZ on cerebral ischemia in rats induced by Ferric chloride2.1Effect of DBZ on the neurological symptoms score and the infraction area in rats with MCAOObject:To study the effect of DBZ on the neurological symptoms score and the infraction area in rats with MCAO.Methods:The MCAO model in rats was induced by Ferric chloride, the neurological symptoms were scored at different time (ischemia6h,24h), the size of cerebral infraction were determined by TTC staining.Results:Model rats showed the severe neurological damage and the large infraction area. All DBZ groups decreased the scores of neurological symptoms, but not so much. DBZ 18mg/kg and54mg/kg reduced significantly the infraction area.Conclusion:DBZ could relieve neurological damage.2.2Effect of DBZ on the contents of LD, LDH, SOD and MDAObject:To study the effect of DBZ on the contents of LD, LDH, SOD and MDA.Methods:The MCAO model in rats was induced by Ferric chloride, the contents of LD, LDH, SOD and MDA were assayed by chemical colorimetry.Results:The contents of LD, LDH, SOD and MDA changed. The LD content increased, the LDH content increased significantly in model group. DBZ6mg/kg could reduce significantly the LD content. All DBZ groups could decrease the LDH content. The SOD content decreased, the MDA content increased significantly in model group. DBZ18mg/kg and DBZ54mg/kg could increase significantly the SOD content.Conclusion:DBZ could scavenging free radicals and relieve the oxidative damage and the acidosis damage after ischemia.3Effect of DBZ injection an oral on cerebral ischemia in rats induced by Ferric chloride3.1Effect of DBZ on the neurological symptoms score and the infraction area in rats with MCAOObject:To study the effect of DBZ on the neurological symptoms score and the infraction area in rats with MCAO.Methods:The MCAO model in rats was induced by Ferric chloride, the neurological symptoms were scored at different time (ischemia6h,24h), the size of cerebral infraction were determined by TTC staining.Results:Model rats showed the severe neurological damage and the large infraction area. DBZ injection36mg/kg decreased greatly the scores of neurological symptoms. DBZ injection18mg/kg reduced significantly the infraction area.Conclusion:DBZ injection could relieve neurological damage.3.2Effect of DBZ laevo isomer and DBZ dextro isomer on the neurological symptoms score and the infraction area in rats with MCAOObject:To study the effect of DBZ laevo isomer (DBZ Ⅱ)and DBZ dextro isomer (DBZ Ⅰ)on the neurological symptoms score and the infraction area in rats with MCAO.Methods:The MCAO model in rats was induced by Ferric chloride, the neurological symptoms were scored at different time (ischemia6h,24h), the size of cerebral infraction were determined by TTC staining. Results:Model rats showed the severe neurological damage and the large infraction area. DBZ I injection18mg/kg decreased greatly the scores of neurological symptoms after ischemia6h,DBZ I injection36mg/kg decreased greatly the scores of neurological symptoms after ischemia6h,24h. DBZ I injection36mg/kg and DBZ II36mg/kg reduced significantly the infraction area.Conclusion:DBZ Ⅰ injection and DBZ Ⅱ injection could relieve neurological damage.4Effect of DBZ laevo isomer and DBZ dextro isomer on cerebral ischemia in rats by the suture-occluded method4.1Effect of DBZ laevo isomer and DBZ dextro isomer on the neurological symptoms score and the infraction area in rats with MCAOObject:To study the effect of DBZ Ⅰ and DBZ Ⅱ on the neurological symptoms score and the infraction area of the nerve cells in rats with MCAO.Methods:The MCAO model in rats was by the suture-occluded method, the neurological symptoms were scored at different time (ischemia6h,24h), the size of cerebral infraction were determined by TTC staining.Results:Model rats showed the severe neurological damage and the large infraction area. DBZ Ⅰ36mg/kg and DBZ Ⅱ72mg/kg decreased greatly the scores of neurological symptoms after ischemia6h,DBZ Ⅱ36mg/kg decreased greatly the scores of neurological symptoms after ischemia6h,24h. DBZ Ⅰ18mg/kg and DBZ Ⅱ36mg/kg, DBZ Ⅱ72mg/kg reduced significantly the infraction area.Conclusion:Both DBZ Ⅰand DBZ Ⅱ could relieve the ischemia damage of nerve cell.4.2Effect of DBZ laevo isomer and DBZ dextro isomer on acidosis and oxidative damage in rats with MCAOObject:To study the effect of DBZ Ⅰ and DBZ Ⅱon the contents of LD, LDH, SOD and MDA.Methods:The MCAO model in rats was by the suture-occluded method, the contents of LD, LDH, SOD and MDA were assayed by chemical colorimetry.Results:The contents of LD, LDH, SOD and MDA changed. The LD content increased, the LDH content decreased significantly in model group. DBZ Ⅰ18mg/kg, DBZ Ⅰ36mg/kg and DBZ Ⅱ72mg/kg could reduce significantly the LD content. DBZ Ⅱ18mg/kg and DBZ Ⅱ36mg/kg could increase the LDH content. The SOD content decreased, the MDA content increased significantly in model group. DBZ Ⅰ72mg/kg and DBZ Ⅱ18mg/kg,DBZ Ⅱ36mg/kg could increase significantly the SOD content. DBZ Ⅰ18mg/kg, DBZ Ⅰ 36mg/kg and DBZ Ⅰ72mg/kg could reduce the MDA content.Conclusion:DBZ Ⅰand DBZ Ⅱcould scavenging free radicals and relieve the oxidative damage and the acidosis damage after ischemia.5Effect of DBZ on the content of inflammatory cytokines in rats with MCAOObject:To study the effect of DBZ on the content of IL-1β,IL-6,IL-8and TNF-α.Methods:The MCAO model in rats was by the suture-occluded method, the contents of IL-1β,IL-6, IL-8and TNF-α were assayed by ELISA.Results:The contents of IL-1β,IL-6, IL-8and TNF-α all increased.. DBZ18mg/kg could reduce significantly the contents of IL-1β,IL-6and IL-8. DBZ36mg/kg and DBZ72mg/kg could reduce the IL-6content.Conclusion:DBZ could inhibit inflammatory reaction.ConclusionIn conclusion, DBZ, which could relieve neurological damage and the acidosis damage, scavenging free radicals, inhibit inflammatory reaction, has the protective effects on cerebral ischemia injury in rats. DBZ injection is more effective than DBZ oral. Both DBZ Ⅰand DBZ Ⅱhas the protective effects as well as DBZ mesomer on cerebral ischemia injury in rats. |
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