| Objective: This dissertation intends to explore and investigate the effect and mechanism of PRP treatment for OA by observing the contents of cytokine IL-1, IL-6 and TNF-α in synovial fluid of the rabbit knee osteoarthritis(KOA) animal models and the articular cartilage tissue sections.Methods: Selecting 32 healthy New Zealand white rabbits as the experimental subject and dividing them into 4 groups at random. Each group has 8 samples. Group A:blank control group; Group B: model group; Group C: PRP treatment group; Group D:Drugs(sodium hyaluronate injection) treatment group. Group A simulates modeling(fake models). Group B, C and D start inducing to establish KOA rabbit animal models in terms of Hulth method. Rabbit KOA mid-term animal models are to succeed after six weeks then proceed to treatment. Group C adopts the twice Centrifugal Process to manufacture PRP then starts its intra-articular injection treatment once in 3 weeks for a total of 2 times. Group A and B are injected with the same amount of normal saline that equals to Group C at the same time. Group D are given the injections of sodium hyaluronate intra-articular injection treatment, once a week for a total of 5 times. After6 weeks of treatment, the animals are to be executed for the following analysis: Firstly,general observation on the situation of the cartilage; Secondly, detection on the contents of IL-1, IL-6 and TNF-α from the extracted synovial fluid according to the ELISA method. Then conducting the one-way ANOVA on the outcomes of the contents of IL-1,IL-6 and TNF-α by utilizing SPSS19.0 statistical analysis software. Lastly, turning the incised articular cartilage tissues into microscope sections by the means of collagen type II immunohistochemical and HE staining. Observing the sections under microscope in which the results are to be evaluated by the standard of Mankin’s Evaluation Table.Results: There are a total of 32 animals serving as the experiment subject. 2 of them were found death due to their unbearable conditions under the surgery. The complement was made accordingly from the back up group and the rest were generally in good conditions during the test. 6 weeks after the treatment, the test results show that the contents of IL-1, IL-6 and TNF-α in synovial fluid of the tested animals were the lowest in Group A and the highest in Group B. Groups C and D were in between. By the comparison of the average contents of the IL-1, IL-6 and TNF-α between Group A and Group B, the difference has statistical significance(P <0.05), which advises that the tested joint of the animal models had degenerative changes. By the comparison of the average contents of the IL-1 and IL-6 between Group B and Groups C, D, the difference has statistical significance(P <0.05), which indicates that, after the treatment, synovialand cartilage inflammation of the tested animals in Groups C and D had been mitigated or suppressed. By the comparison of the average contents of the TNF-α between Group B and Groups C, D, the difference has no statistical significance(P >0.05). By the comparison of the average contents of the IL-1, IL-6 and TNF-α between Group C and Group D, the difference has no statistical significance(P >0.05), which demonstrates that PRP and sodium hyaluronate injection have produced good effect on the mid-term KOA.Conclusions: ⑴PRP can lower the contents of cytokine IL-1 and IL-6 of the OA mid-term synovial fluid, but its effect on the cytokine TNF-α is unobvious. ⑵PRP can mitigate the inflammation of joint and prevent, suppress or reverse the diseasing process of OA to alleviate the symptoms and to improve joint functions. ⑶PRP, as a self-extracted formulation, is safe, effective, non-toxic, non-rejection reaction and has no side effects. Apart from that, it is convenient to produce. To sum up, PRP has a prospect of widespread application on OA treatment. |
PDF Full Text Request