Comparison Of Cytomegalovirus Detection Methodologies In Clinical Specimens And Their Use In Pediatric Cytomegalovirus Infection

Objectives:1, Comparative study of the detection rate for human cytomegalovirus(HCMV)-DNA in the different types of specimens, such as blood, urine and breast milk.2, To.discuss the diagnostic value for HCMV infection by fluorescence quantitative polymerase chain reaction(FQ-PCR) or ELISA method.3, To analyze the correlation between HCMV infection and gender or age.4, To explore the guiding significance of breast feeding by studing the HCMV-DNA positive rate of breast milk.Methods:Selecting 378 suspected HCMV infected infants from the pediatric and pediatric hospitalized children of the Jinxiang People’s Hospital in July 2014 to June 2015 as the research object in the experimental group. And according to the age, the infected infants were divided into two groups: the newborn group(0 to 28 days, 116 cases) and the infants group(29 days – December, 262 cases). The 230 cases were male, and female 148 cases.In addition, 120 newborn infants without HCMV infection were selected as the control group. The blood, urine and corresponding breast milk of the infants or neonates were collected respectively. Then the DNA was detected by fluorescent quantitative polymerase chain reaction(FQ-PCR) and the protein was analized by enzyme-linked immunosorbent assay(ELISA).Results:1, We confirmed that the detection rate of HCMV-DNA in newborn group, the breast milk is the highest, then the blood, and the urine is the lowest. Whereas, in infants group the urine is the highest.2, The urine HCMV-DNA detection rate by fuorescent quantitative PCR were significantly higher than the serum HCMV-Ig M detection rate by enzyme-linked immunosorbent assay(p<0.01).3, The selected samples were tested at different ages and genders, and the correlation between HCMV infection rate and age was statistically significanttest. However, the HCMV infection rate has no relationship with genders.4, In 127 cases of children who were breast by HCMV-DNA positive milk, 92 cases were also detected urine HCMV-DNA positive(74.8% positive rate); and in 59 cases of children who were breast by HCMV-DNA negative milk, 31 cases were detected urine HCMV-DNA positive(25.2% positive rate). There are significant difference(chi-square =7582, P < 0.01).Conclusion:This study confirmed that instead of blood detection of cytomegalovirus, urine HCMV-DNA detection can be used to screen HCMV by studing the detection rate of HCMV-DNA in urine. In addition, by comparative studing the detection rate of serum Ig M antibody detection by using enzyme detection and HCMV-DNA detection by using fluorescence quantitative PCR assay, the fluorescence quantitative PCR method has a higher detection rate and the clinical using of HCMV diagnosis by fluorescence quantitative PCR method has more advantages and value. Then, by the comparison study of different age and gender in children for the cytomegalovirus infection rate, we found the cytomegalovirus infection has relationship with age, but has no correlation with gender; in addition, although the milk itself is sterile, but it can spread a variety of pathogens. Many infectious diseases can be directly transmissed by breastfeeding, so the safety of breastfeeding is a serious question.