The Physicochemical Characteristics Of Bitter Polysaccharides And The Improvement Of Exercise Fatigue And Chronic Fatigue Syndrome

Objective:In this study, SF and CFS for animal models, explore the effects of SF and CFS on the body and through additional HPP on regulation of resist fatigue effects on animals. Preliminary analysis of SF and the effects of CFS on sport ability of body, also provide experimental evidence for development of HPP of medicinal value.Experiment method:1、Preparation and physico-chemical properties of HPP identification methodThrough centrifugal, and water dissolved alcohol sank, and Sevage except protein, method preparation get of HPP rough polysaccharide, again through Qualcomm cellulose column filter have HPP fine polysaccharide. Sugar content of protein and miscellaneous protein and nucleic acid were detected, By UV-visible spectroscopy, and infrared spectrometer, and HPLC, and Kjeldahl Apparatus, and ESEM, and AFM physical and chemical properties identified.2、HPP’s influence on sports fatigue testing methodWith 5 week established small rat SF model, irrigation stomach different dose of bitter dish polysaccharide protein water solution, on SF model determination animal behavior learn aspects of index, and organization biochemical index and urine liquid metabolism group learn index, organization biochemical index respectively in force exhaust movement immediately, and movement 24h recovery two species situation Xia determination, blood biochemical index has Bla, and BUN, and CK, skeletal muscle and liver biochemical index has glycogen content, and MDA content, and GSH-PX activity.3、HPP on CFS testing methodWith 4 weeks with construction of the animal model of CFS, the CFS model is multi-stage urine collection and determination of animal behavior indicators, GC-TOF-MS metabolomics technology on animal every stage indicator indexes compare and identification. Animal behavior indicators separately for body weight, drinking, feeding, water maze, tail suspension test, field experiments; urine metabolic indicators separately for routine urine comparison, total ion, PCA differences in analysis and OLPS-DA analysis, screening and identification of metabolites.Results:1、Results of physico-chemical characterization of HPPHPP the typical structure of polysaccharides, monosaccharide mainly composed of D-mannose, L-rhamnose and D-glucuronic acid and D-glucose, D-galactose and L-arabinose formed. In the detection of amino acids of the HPP, detect Cys, Leu, Tyr, Lys, His.2、HPP delaying sports fatigue test resultsAnti sports fatigue, bitter vegetable polysaccharide on exhausted mice’s CK, MDA, glycogen recovery was inhibited, on exhausted mice skeletal muscle, liver, GSH-PX activity has improved. Reduces the body of mice with exhaustive 24h CK and MDA in mouse liver, promote on exhausted mice 24h muscle glycogen and hepatic glycogen recovery, increasing exhaustion of 24h GSH-PH activity in the liver of mice. Concentration of polysaccharide on the BUN, Bla and exhaustive in mouse skeletal muscle MDA, muscle glycogen without any effect. Inferred that HPP has a good effect on the SF.3、HPP improve CFS test resultsCFS bitter vegetable polysaccharide on the CFS has a relief effect. CFS caused by eating, drinking drinks, weight, weight loss, growth retardation, dry coarse hair, exploring capacity, low learning ability, memory and other symptoms, have a very good effect. In urine testing, KET、NIT is not diagnosed positive. GLU, WBC, WBC, URO, BIL, BLD, PRO data were reduced, Improve urine index of efficacy. CFS group compared with silent group out of 12 different metabolites, were:Malic acid, fumaric acid, Palmitic acid, sphingosine,2, fructose-6-phosphate and a-ketoglutaric acid, gluconic acid, tartaric acid, malonic acid,21-hydroxy enol-one pregnancy, maleamic acid, shikimic acid, acetyl-1-ornithine. For disorders metabolism system is mainly caused by CFS:TCA cycle; CFS group compared with HPP treated group out of 8 different metabolites:Salicylic acid, D-arabitol, Stearic acid, Palmitic acid,2-Hydroxy pyridine, Salicin, Sphingosine, L3-methyl alcohol.HPP affects the metabolic system on the CFS are:GPI and Glycerophospholipid metabolism which are 2 common differences in metabolites (Sheath ammonia-alcohol and Palmitic acid) in relation to the CFS model.has changed in the opposite direction, suggesting that metabolism disorder caused by CFS, HPP focused improvements.Conclusion:Through this research series experiment, proved HPP is a protein polysaccharide combined real, has unique of molecular morphology, and on SF has good of delay role, its mechanism and improve body anti oxidation capacity and sugar metabolism capacity about; while HPP on CFS also has good of improved role, its metabolism mechanism and improved has three acid acid cycle, and sugar base Phospholipid muscle alcohol biological synthesis and glycerol Phospholipid metabolism pathway about.