| Purpose: The main object of this research is to explore the chronic heart failure (chronic heart failure, CHF) heart kidney related mechanisms and to observe the interventional effect of Shen Cao Tongmai Granule to CHF. In order to achieve this objective of this research, to establish the heart failure after myocardial infarction model in rats model, then drench lisinopril and Shen Cao Tongmai Granule to the mice by group to observe the expression of OPN in Myocardial tissue and kidney tissue. To provide the scientific basis for the concept of heart renal pathogenesis of Chinese medicine treatment of chronic heart failure.Material and method:1.To prepare the rat model with chronic heart failure: 60 SPF 12 weeks healthy male SD rats which weight is in 230g～250gwere chosen. 15 of them for sham-operation (open chest line but not coronary artery ligation), the remaining 45 for heart failure model after myocardial infarction by ligation rat left anterior descending coronary artery, underfeed after operation,load exercise. After set up the model and calculated by doppler echocardiography, the left ventricular ejection fraction (EF)of rat is less than or equal 55% ,at the same time of dynamic ecg display chest lead in ST -T change, and accompanied by a significant depression,decreased activity, love to jam togehg, the colour and hair removal, diet reduce chronic heart failure signs can be incorporated into the subject.2.The experimental group and feeding:26 rats with successful chronic heart failure model were randomly divided into model group (8), Chinese medicine group (9) and western medicine group (9). At the same time, 9 rats were randomly selected from sham-operation to build a sham-operation group. Sham-operation group and model group to give regular volume of distilled water, dosage is 3 ml/time, 2 times/day; without drugs; Chinese medicine group was given and grass through vein granule lavage, dosage of crude drugs is 9.2 mg per kilogram per day; The western medicine group was given lisinopril lavage, dosage of crude drugs is 1.5 mg per kilogram per day . Do CDIF for these rats after 28 days continuous feeding, kill all the rats after test and take samples of blood, heart, kidney tissue for inspection.3.Detection method:To determine the LVID (Ed), LVID (es), EF and FS of each group by Doppler echocardiography. To calculate the serum BNP concentration by ELISA method. To observe the OPN and OPNmRNA expression level in myocardial tissue and kidney tissue of Sham-operation group, model group, Chinese medicine group and western medicine group by Real - time PCR technology and immunohistochemical method, and to observe changes of shape and structure of myocardial tissue under the electron microscope.Results:1. Compared with control group, the LVID(ed)、LVID(es) of model group were increased (P <0.01), and EF and FS were lower (P < 0.01); Compared with model group, LVID (Ed) and LVID (es) of Chinese medicine and western medicine were lower, and EF,FS of Chinese medicine, western medicine group were higher. The marked differences were statistically significant (P < 0.01). There were no significant difference between Chinese group and western group, it is no statistical significance.2.1n cardiac and renal tissues, compared with Sham-operation group ,the OPNmRNA expression in drug intervention group and model group were increased significantly, P =0.000 (P < 0.01); Compared with model group , the OPNmRNA expression in drug intervention group was lower, it has statistical significance (P < 0.01); No statistical differences between Chinese and western group, P = 0.878 (P > 0.05).3.Compared with Sham-operation group , OPN protein expression in cardiac and renal tissue of model group was significantly elevated. There were clear differences between these two groups, P = 0.000 (P < 0.01), OPN protein expression differences in cardiac and renal tissue were not obvious between Chinese and western group, there is no statistical significance (P =0.804; in the myocardial tissue renal tissues (P = 0.669).4.BNP expression of model group increased significantly, it is about 2.26 times of control group. Compared with control group (P = 0.000, (P < 0.01), there were significant differences between the two groups. With drug intervention, the BNP concentrations significantly decreased (compared with model group P < 0.01); The were no statistical difference between Chinese group and western group (P > 0.05).5.Observed under electron microscope,control the myocardial muscle fibril arrange compact,neat, boundary between light and shade with clear and tidy, mitochondrial quantity is rich, the substrate is arranged closely, neat ridge are arranged in dense, form is clearly visible,glycogen particles full rich; Model group disordered arrangement of myocardial fibers, and is markedly swollen mitochondria, the ridge structure disorder or disappear, show clear air ball,and the number of glycogen corresponding lower; Compared with the model group, the mitochondria increased more than that of the model group, and there were still abnormal structures such as swelling deformation and ridge disorder in the myocardium of the traditional Chinese medicine group and the western medicine group.Conclusion:1. The method of build a model of chronic heart failure after myocardial infarction by ligation of left anterior descending coronary artery of rats and , underfeed after operation, load exercise is feasible and can be applied to studies of chronic heart failure.2. Compared experimental data, it has been proved that the Shen Cao Tongmai Granule can inhibit myocardial and renal fibrosis and refactoring by cut the expression of OPN in heart and kidney tissue.3. Through the research, it has been proved that the Shen Cao Tongmai Granule can achieve the aim of treatment for common treatment of heart and kidney,and it is safe and effective for clinical application of treatment in chronic heart failure, the treatment effect was equal to lisinopril. |
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