The Identification And Screening Of Migration Family From The Same Ethnic

ObjectiveBy researching and studying the Xibo nationality,who has clear mi gratory history of Shenyang and Xinjiang,carried out migration sampl es,and then on the sampling of the Xibe Nationality male volunteers were 22 Y-STR loci detection to finish the racial identity analysis.I dentification of genetic relationship for migratory populations can p rovides data and basis for subsequent studies on migration related fa ctors and geographical environment of ethnic features,also the estab lishment the Y-STR database of Xibe,inrich Xibe genetics data,provi de reference data for forensic identification of Xibo people in other areas and also for the study of human genetics and other studies.Methods1.Sampling by random method.The surname of Wu?Guang?He?Tong?E?Han is a large number of Xibe groups,possibility of meeting the s ampling number.According to the name in accordance with the surname w riting stroke order compiled 1?6 groups,six random sequence number s were generated,to random sample names.2.Through the medical ethics audit of General Hospital of Guangz hou Military Command,and after with the consent of the volunteers th emselves signed the informed consent,Selection and investigation of Xibe population in the Shenyang and Xinjiang.Then sampling design and field collected,including peripheral venous blood,facial two-dimen sional and three-dimensional image data and CT data acquisition.3.Adlai solution type whole blood genomic extraction kit for whole blood genomic DNA extraction,and the blood filter paper,in two ways of blood sample preservation.4.Amplification of PCR:GeneAmp PCR system9700PCR(AB company,USA),PPY-23 kit amplification of Y chromosome 22Y-STR(DYS576,DYS3891,DYS448,DYS389?,DYS19,DYS391,DYS481,DYS549,DYS533,DYS438,DYS437,DYS570,DYS635,DYS390,DYS439,DYS392,DYS643,DYS393,DYS458,DYS385,DYS455,YGATAHA).5.Electrophoretic separation and genotyping:3130*1 DNA genetic analyzer separated by capillary electrophoresis(AB company,USA)and use GeneMapper ID Software v3.2 software for genotyping read.6.Statistics and analysis:The standard of blood glucose and blood lipid reference to the local hospital as they have they own method and machine to detection.Excel 2013 to input the data and SPSS 18.0 statistical software for statistical analysis,the age,gender,region and all the blood glucose and lipid data,the measurement data of normal distribution said to mean±standard deviation(x±SD),use the independent samples t test,between the different regional groups,blood glucose,blood lipids outliers difference holds concurrently with chi-square test or the Fisher exact probability,P<0.05 means the difference was statistically significant.According to the results of the genotype,Excel used as direct method for counting allele frequencies and haplotype frequencies,according to Nei method,the haplotype diversity(haplotype-diversity,HD)gene diversity(gene diversity,GD=n(1??Pi2)/(n-1).(Pi is the allele or haplotype frequencies,n is the number of samples).AMOVA analysis was carried out using Arlequin V3.5.1.3 software,the calculation of genetic distance of Rst matrix between the Xibe population from two areas.Results1.Completed the epidemiology Design and choose the crowd(area,family,etc.),finished volunteer recruition and collection?transportation and storage,135 blood samples?digital facial photos?3D facial data and brain CT data were obtained 121 respectively,and preliminary data processing.There is no statistically significant difference between the two groups between different gender,age(P>0.05),it' s comparable.There was statistically significant difference between BMI values(P<0.05).The abnormal rate of GLU showed a Statistical significant between different area(P<0.05),while the TC,HDL,LDL and TG showed no difference(P>0.05).2.Both Shenyang and Xinjiang Xibo groups in CT soft DBE,3D-DBE,CT bone DBE had significant differences;CT soft ABC,CT soft DBE,3D-ABC,3D-DBE,CT bone DBE has significant difference between male and female;Dividing the boundary by the age of 50,CT soft DBE and 3D-DBE two items had significant difference.3.22 Y-STR loci showed a general polymorphism detection between the two groups Xibe,between the two regions was not observed haplotype sharing.Conclusion1.The migration groups of Xibo has a significant difference in blood glucose,considering the influence of environment and diet,People's attention on blood glucose and blood lipid should be promoted,meanwhile,the healthy lifestyle and reasonable diet should be promoted to improve their health rates.Regional differences apparently.2.CT ABC,3D-ABC CT,soft bone ABC can be used as a sign of Xibe ethnic characteristics measurement,Soft tissue DBE arc with facial rejuvenation,as one of the younger pointer,it is of great significance to guide the young facial contour modification.3.Using Y-STR to determination of family unity,through the comparison of the two male 22 genetic loci,difference larger that can not be two people family the same conclusion.