Health Risk Of Heavy Metals In Farmland And Its Correlation With Soil Microbial Richness In Shandong Area

Shandong is a relatively rich mining area.For economic development,more and more mining areas are being explored,resulting in more farmland surrounded by mining areas.The health risk caused by heavy metal pollution has attracted worldwide attention in agricultural soil.And the research on accumulation of heavy metals in soil plant system is based on human health risk assessment.According to the Shandong Provincial Institute of Geological Survey data in Shandong province,we selected numbered ZB?RZ?YT?ZZ four abnormal content of heavy metals in soil areas.According to the second standard of sampling points of soil heavy metal pollution assessment of soil environmental quality of China and Shandong Province,we detected the content of As?Cd?Cr?Cu?Hg?Pb?Ni?Zn,analyzed the pollution situation of corn and wheat seeds were analyzed through the national and EU food safety standards as the basis.It turns out that the largest degree of pollution contribution in these areas are Hg and Cd,the largest health risk value is Cr.The health risk assessment of wheat seeds in these ares,THQ of Cd values are greater than 1,indicating Cd in wheat seeds has caused a great bad impact on human life.The corn seeds in YT area,only the THQ value of Hg is greater than 1,the single factor pollution value in other parts is less than 1,the value of compound pollution is also less than 1,which will not cause harm to the lives of local residents.Another focus of this study is to analyze the abundance of soil microbial species by using 16S rRNA high throughput sequencing.Microbial sequencing is mainly in numbered BS,WL,ZY,LL and QH.The PCA analysis showed that ZY and WL area have similar bacterial community composition of,and the great differences of BS and LL composition.Abundance of the soil microbial species was analysed with 16S rRNA gene.The levels of As,Cr,Hg,N,Ni,Pb in soil,and the soil pH were found to be significantly correlated with the abundance of bacteria according to principal component analysis(RDA).In other words,the heavy metals,non-metallic elements and soil properties combined to affect the abundance of soil bacteria.Most of the species range found in our study include Actinomycetes and Proteobacteria,The Neemelo index in WL area the pollution level are the smallest.However,the Actinomycete abundance is the highest among all the samples,followed by the Proteobacteria.The sample of BS has the highest pollution level in all the samples,and the minimum variation rate of the strain.The results provide a scientific basis for understanding the soil pollution in this area.The health assessment is also convenient for people in the area to understand the health status of crops.Whether it is the assessment of soil pollution or the health assessment of the crops,it is important to control the environmental pollution in the future.