Effects Of IGF-1 On Osteogenic Differentiation Of Rabbit Adipose-derived Stem Cells

Objective: To explore the influence of insulin like growth factor(IGF-1)on the proliferation and the osteoblastic differentiation ability of rabbit adipose-derived stem cells(ADSCs).Methods: The adipose tissue was removed from the rabbit neck in aseptic condition.Isolate adipose-derived stem cells from the adpose tissue by the method of collagenase digestion,and identificate CD90 by immunofluorescence technique.Added 0,5,10 and 20ng/ml IGF-1 into osteogenic medium,observed proliferation activity of ADSCs and the content of ALP and calcium ion.The proliferation activity of the ADSCs was detected by the CCK-8,and the osteoblastic differentiation ability was detected by alizarin red staining.Quantitative test of ALP and calcium ion was used to analysis ADSCs osteogenetic differentiation ability? Results: The original and subcultured adipose-derived stem cells were mainly spindle-shaped like fibroblast and proliferated quickly.The immunofluorescence test showed that ADSCs high expresses of CD90,and it could differentiate into osteoblasts and adipocytes.IGF-1 promote the proliferation activity of ADSCs and increase the content of ALP and calcium ion.The more concentration of IGF-1,the higher proliferation activity of ADSCs and the more content of ALP and calcium Conclusions: IGF-1 can promote the proliferation and osteogenesis differentiation ability of rabbit ADSCs.