The Study Of The Firing Rhythm Of Neuron Modeland Blood Oxygenation Metabolism With Transcranial Ultrasound Stimulation

Transcranial magneto-acoustical stimulation(TMAS)is a kind of brain stimulation technology that can modulate neuronal activities.It uses ultrasonic wave within a magnetostatic field generates an electric current in an area of interest in the brain to modulate neuronal activities.It has the advantage of noninvasive,high spatial resolution and penetration depth.However,the effect of TMAS on the neurons remains unknown.Therefore,the study of TMAS has great significance.The paper is divided into two parts:theoretical research and experimental research.The theoretical research mainly studies the neuronal spike-frequency adaptation or the firing rhythm under different models on the basis of TMAS.The experimental aspects mainly studies the blood oxygenation metabolism under transcranial ultrasound stimulation(TUS).Firstly,the paper studies the effects of ultrasonic intensity,magnetostatic field intensity,ultrasonic frequency,duty cycle and modulation frequency under TMAS on the neuronal spike-frequency adaptation based on the Ermentrout neuron model.These results of the study can help to reveal the mechanism of transcranial magneto-acoustical stimulation on the neuronal spike-frequency adaptation,and provide a theoretical basis for the treatment of neurological disorders.Secondly,the paper studies the effects of the amplitude frequency ratio and the current density under TMAS on the firing rhythm of the amplitude of the membrane potential,the membrane potential interspike interval and the membrane potential firing rate based on the AHH neuron model.The results show that the effect of TMAS on the firing rhythm of neurons can help to explore the application of TMAS in the treatment and rehabilitation of neurological diseases.Finally,the paper studies the blood oxygenation metabolism under TUS.The purpose of the current study was to evaluate the effect of low-intensity TUS on the blood oxygenation metabolism activation using intrinsic signal optical imaging(ISOI).Low-intensity TUS was applied to modulate mouse motor cortex and caused facial muscle contraction and whiskers movement concurrently with ISOI monitoring of changes indeoxyhemoglobin(HbR)through intact skull.The experimental results show that TUS causes a dynamic decrease in the HbR,reaching a peak response at the time of 2.6 s after stimulus onset and recovering to baseline.Furthermore,the results also show that the peak value of HbR in the parenchyma region is significantly lower than those of the venous region.These results demonstrate that low-intensity TUS can induce change of blood oxygenation metabolism,potentially explaining changes in sensitivity to stimulation with TUS.The above research results reveal the mechanism of TMAS and TUS in the biological nervous system and provide a theoretical basis for the treatment of neurological diseases.