The Analysis Of Soil Bacterial Communities Diversity In The Longmen Mountain Seismic Zone From Yinchanggou Area

The 8.0 Ms Wenchuan earthquake and the 7.0 Ms Lushan earthquake occurred successively in the surrounding area of Longmen mountain seismic zone in nearly a decade,but few reports about soil microbes in the earthquake stricken area.And there are few studies about the relationship between soil physical and chemical properties,vegetation diversity and microbial community diversity,and how to restore fragile habitats after the earthquake.Therefore,in this study,the soils of three vegetation types such as mixed forests,bamboo forests and pine forests in the Yinchanggou area of the Longmen Mountain seismic zone were collected as research objects.Density gradient gel electrophoresis(DGGE),Illumina sequencing,and BIOLOG Ecosystem(ECO)culture were used to study the diversity of soil bacteria 16 S rRNA(V6 ~ V8)community and soil 16 S rRNA(V3-V4)Community Diversity and Metabolic Function Diversity of Soil Microbial Communities in Bacteria.At the same time,combined with soil physicochemical properties,the relationship between soil,vegetation types,and microbial community diversity in the study area was thoroughly discussed.The results of DGGE sequencing showed that Rhizomicrobium,Streptomyces,Halomonas,Lelliottia,Bryobacter,Thiofaba,Halomonas,Labilithrix,Fimbriimonas,Paludibaculum and Candidatus Solibacter were mainly found in the soil of Yinchanggou area of the Longmen Mountain seismic zone.The ten Genus bacteria belong to the four levels such as Proteobacteria,Acidobacteria,Actinobacteria,and Armatimonadetes,among which Proteobacteria are the dominant bacterial groups in the soil of the study area.There was a difference in the bacterial community diversity in the planted type soil.And the diversity of the bacterial community in the soil of the mixed forestwasthe highest,followed by the bamboo forest and the lowest was the pine forest.The results of Illumina sequencing indicated that the soil bacterial in the study area could belong to 33 Phylum,90 Class,132 Order,153 Family,151 Genus,and 51 Species.In addition,at the level of phylum classification,the relative abundance of bacteria was Proteobacteria,Actinomycota,Acidobacilli,and Chloroflexi;in terms of bacterial community diversity,3 types of planting are showing differences.The overall trend was the highest in the mixed forest,the middle in the bamboo forest,and the lowest in the pine forest.The prediction of the metabolic characteristics of the main functional flora found that the relative abundance distribution of functional flora in three soil bacteria was different in different metabolic pathways.BIOLOG ECO Plate Culture results showed that the main types of carbon sources used by soil bacterial communities are different in mixed forests,bamboo grove,and Pine forest,including amino acids,esters,alcohols and amines,carbohydrates,amino acids,esters and alcohols,carbohydrates,esters,amines,and acids.In addition,there are some differences in its metabolic function diversity.The pine forest is highest,followed by bamboo forest,mixed forest the lowest.In summary,the results showed that there are certain differences in the genetic diversity and carbon source metabolism characteristics of soil bacterial communities under different vegetation types.The root exudates of different plants were the cause of this difference,and the physiological activities of soil bacteria could be also affect soil fertility.The interaction between plants and bacteria caused differences in the types and contents of bacteria in different soils tested.Thus,there are differences in the genetic diversity and metabolic functional diversity of bacterial communities.Therefore,this study provided a scientific basis for the restoration of the ecological environment of the Longmen mountain seismic zone after the earthquake and lays a theoretical foundation for maintaining the sustainable development of the ecosystem in the study area.