Identification Of Important Physiological Functional Genes Of Mythimna Separata And Antheraea Pernyi Based On Transcriptome Sequence Analysis

The emergence of high-throughput sequencing technology has made it possible to understand the transcriptional composition of a species or a particular tissue from an overall level,and has now become the mainstream technology for the study of mRNAs lacking genomic information species.It also provides a way for rapidly identification of the functional genes of these species.In this paper,the de novo transcriptome sequencing of M.separate adult heads was completed,and the gene expression of heads of Mythimna separata was revealed.The circadian clock related genes,the melanin synthesis pathway related genes,and the non-receptor protein gene family of olfaction were identified.Based on the transcriptome sequencing data,genes involved in the ecdysone synthesis pathway in Antheraea pernyi were also identified.Mythimna separata is an important agricultural pest with wide distribution and strong ability to migrate.However,little is known about the molecular regulation mechanisms of the physiological and behavioral stress responses of armyworm migration.In this paper,we took a transcriptomic approach to characterize the gene network in the adult head of M.separate.A total of 63,499 transcripts were generated by sequencing,and 46459 unigene(1153 bp for N50)were further assembled.Of these,22,334 had annotation information in the database.We found that in the transcriptome data of adult heads,the genes involved in signal transduction were the most abundant,and a total of 937 signal transduction unigene were annotated to 22 signal pathways.Based on the transcriptome data obtained,the circadian clock,melanin synthesis,and non-receptor protein of olfactory gene families were then identified.The transcriptome data fully revealed the gene expression of heads of the adult M.separata.These annotated genes laid the foundation for further functional research in the future.During the growth and development of insects,the synthesis and secretion of ecdysone in the prothoracic gland plays a crucial role.For example,ecdysone can relieve the diapause status of pupae.Recently,our laboratory has found that the imidazole compound KK-42 can delay the release of diapause of pupae.Both of these physiological changes are related to the ecdysone synthesis pathway.However,at present,we know very little about the genes involved in the ecdysone synthesis pathway in A.pernyi.Therefore,based on the transcriptome data of the prothoracic gland in A.pernyi previously completed in our laboratory,this paper identified genes involved in the ecdysone synthesis pathway in theprothoracic gland in A.pernyi,including Neverland,Spook,Phantom,Disembodian,and Shadow.The results of RT-PCR confirmed that the Spook,Phantom and Shadow genes were all expressed in the prothoracic gland.The identification of genes related to ecdysone synthesis pathway laid the foundation for further functional studies.