Comparative Genomic Analysis Of CAZymes In Plant Saprophytic,pathogenic And Endophytic Fungi

As a large group of eukaryotes,fungi play an indispensable role in the material cycle of nature.Different types of fungi play different roles in the material cycle.For example,some mites are available for human consumption,but some fungi have invasive and damaging effects on crops,such as phytopathogenic fungi.Therefore,how to make better use of fungi has become the research direction of mycology in recent years.Fungi can produce a large number of carbohydrate active enzymes(CAZymes),which are a prominent family of proteins in the growth and development of fungi,which may be closely related to the lifestyle of fungi,and also degrade lignocellulose to solve energy.An important enzyme system built by the crisis green bio-platform.In this study,we analyzed the composition and content of CAZymes in fungi from saprophytic,pathogenic and endophytic fungi by comparative genomics,so as to understand the relationship between the number and diversity of CAZymes gene coding and fungal nutrition and reveal three types.The different degradation ability of fungi to lignocellulose provides feasibility for finding microorganisms that efficiently degrade biomass.The main research contents and results of this paper are as follows:1.Firstly,in this study,the original sequences of 9 endophytic fungi in the research group were analyzed for assembly and splicing,and gene prediction.On the basis of this,CAZymes screening was performed together with 88 fungi of known sequences in the database.The results showed that the endophytic fungus JR14 strain contained the highest number of CAZymes,up to 1938,among the 97 fungi that contained various living and nutrient patterns of saprophytic,pathogenic and endophytic fungi.2.Secondly,the identification and comparison of CAZymes encoded by fungi with different lifestyle nutrition patterns or host infestation methods can better understand the life pattern and infection pattern of fungi.This study systematically identified CAZymes of 97 fungi of saprophytic,pathogenic and endophytic fungi.The comparative genomics analysis found that there were large differences in CAZymes among different types of fungi.Endophytic fungi contain more abundant CAZymes than saprophytic and pathogenic fungi.In addition,the results of this study demonstrate the expansion of the CAZymes gene family in fungi and reveal itsrelationship to fungal nutrition and host specificity,laying the foundation for subsequent research.At the same time,a detailed analysis of a strain of endophytic fungus JR14,which is highly efficient in producing CAZymes,found that JR14 strain has more abundant CAZymes than the enzyme strains that mainly produce biodegradable biomass in the industry.Enzyme systems that degrade biomass materials offer new goals.3.Finally,the effects of transposon and transcription factors on the lifestyle nutrition model of fungi were explored,and comparative analysis of virulence factors was carried out.The results showed that there were no differences in transposon and transcription factors among the three types of fungi,but there were significant differences between saprophytic fungi and pathogenic fungi and endophytic fungi in terms of virulence factors,while pathogenic fungi and endophytic fungi did not.difference.In this study,the genome-wide sequence of 97 fungi was used as the research object,and the content of CAZymes in the three fungi was analyzed by comparative genomics.At the same time,the transposon,transcription factors and virulence factors in the three fungi were systematically analyzed for better results.Understand the physiological processes of different nutrient-based fungi and reveal the ability of three types of fungi to degrade lignocellulose,and finally provide a theoretical basis for screening microorganisms that efficiently degrade biomass.