The Distribution Of Tick And Common Tick Borne Diseases In Quzhou City

Objective:With the development of globalization,transportation and tourism,the harm caused by tick borme diseases is not only a health problem,but also an economic,social and political problem.According study of the epidemic and distribution of tick borme diseases in Quzhou City,to provide references for the practical prevention and control of Quzhou City tick-borne infectious diseases.Methods:It's a descriptive study.The samples conducted from April to November in 2014-2016,once a month.The study sties were Kaihua County and Kecheng District.Seleeted habitats such as indoor,farmland,mountainous,barren slope and grassland to captured rodents.Rodents were trapped by using peanuts as bait from two sites,which is also the peak season for tick dynamic and survival.The Rodent species were identified after captured,spleen specimens were collected and stored at-20?.At the same time,collected ticks,wild animals and domesticated animals.The CDC Investigated of suspected tick-borne disease patients and previous infection persons.Nested PCR amplification with the use of primer designed to amplify a fragment of the citrate synthase gene of Borrelia burgdorferi sensu lato,Rickettsia,Anaplasmataceae and Babesia.A Chi-square test or Fisher's test was used to assess Rickettsia,Anaplasmataceae,Borrelia burgdorferi sensu lato and Babesia infection rates of rodents from two sites respectively.P values<0.05 were considered statistically significant by using SPSS version 16.0.The sequences of these 4 pathogens from two study sites compared to published on Genbank sequences using the BLAST program from National Center for Biotechnology Information Website,and phylogenetic analysis was carried out using the MEGA 5.1 software.Results:During 2014 to 2016,a total number of 603 rodents were captured from study areas.120 rodents were captured form indoor in Kecheng District in 2014 and 23 form indoor,460 outdoor in Kaihua County in 2015-2016.The rodent peaks were in April and from September to December.In Kaihua County,the highest number was captured in farmland,followed by natural villages,woodlands and mountainous areas,and the lowest number was captured on barren slopes.A total of 269 rodents were identified and test,the rodents captured from Kecheng District indoor was 120,the species were Rattus Norvegicus(58.33%)and Rattus flavipectus(41.67%).The rodents captured from Kaihua County indoor and outdoor were 149.The number from indoor was 23,the species were Rattus Norvegicus,Rattus flavipectus,Niviventer confucianus and Niviventer fulvescens.The number from outdoor was 126,the species were Apodemus agrarius,Niviventer fulvescens,Niviventer confucianus,Rattus Norvegicus,Rattusflavipectus,Rattus edwardsi,Rattus bowersi,Microtusfortis and Suneus murinus.591 ticks were collected from wild animals in Kaihua County,including Haemaphysalis longicornis 443 and Rhipicephalus microplus 148.Free-living ticks did not collected in Kaihua County.Free-living and parasitic ticks did not collected in Kecheng District.Used Nest-PCR to detected 269 spleens from rodent.Infection rates for Borrelia burgdorferi sensu lato was 21.56%,The highest infection rates were detected from Rattus Norvegicus,and followed by Niviventer fulvescens.The infection rates among rodents species were different(?2=25.66,P=0.000).The infection rates between Kecheng District and Kaihua County were different,significantly(?2=43.55,P=0·000,OR=9.27,95%CI:4.43-19.39).In 269 spleens,detected infection rate of Rickettsia was 13.38%.The highest infection rates were detected from Rattus Norvegicus,and followed by Niviventer fulvescens.The infection rates among rodents species were not significant difference(?1=2.98,P=0.40).The infection rates between Kecheng District and Kaihua County were different,significantly(?2=9.58,P=0.002,OR=3.30,95%CI:1.50-7.24).The Anaplasmataceae infection rate was 21.19%.The highest infection rates were detected from Rattus edwardsi,and followed by Niviventer confucianus.The infection rates among rodents species were different(?2=16.09,P=0.000).The infection rates between Kecheng District and Kaihua County were different,significantly(?2=0.60,P=0.44,OR=1.26,95%CI:0.70-2.26).The Babesia was only detected three sites form Kaihua County,the infection rate was 3.72%.The highest infection rates were detected from Niviventer fulvescens,and followed by Niviventer confucianus.The infection rates among rodents species were different(?2=17.56,P=0.001).Rodents from 2 study sites were positive for multiple co-infections.Co-infections Rickettsia Anaplasmataceae was detected in Kecheng District and Kaihua County,the infection rates were 1.67%and 0.67%,respectively.Co-infections Anaplasmataceae and Borrelia burgdorferi sensu lato only detected in Kecheng District,the rate was 6.67%.Co-infections Rickettsia,Anaplasmataceae and Borrelia burgdorferi sensu lato only detected in Kecheng District,the rate was 0.83%.Co-infections Borrelia burgdorferi sensu lato,Anaplasmataceae and Babesia only detected in Kaihua County,the rate was 0.67%.A total of 1218 human serum samples were collected,32 of which were positive.The positive rate of Borrelia burgdorferi sensu lato was 2.63%.The difference of positive rate between different genders.The Borrelia burgdorferi sensu lato sequences form were rodents similar to those detected in Tibet,Hunan province and Czech republic,respectively.The sequences of Rickettsia were similar to Jilin province and Australia.The sequences of Anaplasmataceae were similar to Xinjiang,Beijing,Turkey and Mexico.The sequence of Babesia was similar to Xinjiang.The study collected 1218 serum samples from healthy people(Kecheng District 588,Kaihua County 630),the male was 515 and the female was 703.The serum samples were detected antibodies of Lyme disease and Severe fever with thrombocytopenia syndrome.The number of Lyme disease antibodies was 32,and the positive rate was 2.63%.The positive rates of sexes were different(?2=5.50,P=0.019,OR=2.33,95%CI:1.13-4.80).The positive rates of Kecheng District and Kaihua County were different,significantly(?2=3.96,P=0.047,OR=2.08?95%CI:0.99-4.36).The number of Severe fever with thrombocytopenia syndrome antibodies was26,and the positive rate was 2.13%.The positive rates of sexes were different(?2=5.81,P=0.016,OR=2.63,95%CI:1.16-5.95).The positive rates of Kecheng District and Kaihua County were different,significantly(?2=4.81,P=0.08,OR=0.39,95%CI:0.16-0.93).From 2014 to 2016,the county-level general hospitals in two sites were not detected suspected patients of tick borne disease.Conclusion:1.The tick were found in natural environment of Quzhou Ctiy.The pathogens of tick borne disease were detected in ticks and tick host animals.The serum samples from healthy people also detected tick borne disease antibodies.These indicated Quzhou City has the risk of tick borne disease.2.Although hospital surveillance has not found supected tick borne disease patients,Quzhou City medical institutions should continue to strengthen the monitoring of tick borne disease.3.The research on tick-borne diseases should be strengthen.For prevention of people infect tick-borne diseases,should understand the pathogens carrying status of ticks and tick hosts,and the possible exposure opportunities of the population.