Genome-based Transcriptome Of The Recombinant Ar_Han0458 Obtained By Ion Beam Implantation

In order to further obtain and understand the metabolism and transcriptional regulation of the recombinant strain Ar_Han0458 obtained by low-energy ion implantation-mediated exogenous transformation,RNA-Seq and bioinformatics methods been applied.Recombination yeast Ar_Han0458 and original strain As2340 was sequenced at 00 h,24h,48 h,72h,96 h.At the same time,transcription factor analysis was performed on the promoter region of the alternative spliced mRNA.The results of Differentially expressed genes(DEGs)showed that the number of downregulated genes in 24/00,48/00,72/00,96/00,48/24,72/24 was higher than upregulated genes.In others,except for the recombinant strain Ar_Han0458 were equal in 72/48,the number of genes up-regulated was greater than that of down-regulated genes.As the Gene Ontology(GO)enrichment of DEGs showed,there are 23 biological processes,7 cellular components,and 10 molecular functions were shared by As2340 and Ar_Han0458;20 biological processes,6 cellular components,and 3 molecular functions were As2340 Unique;5 biological processes,2 cellular components,and 7 molecular functions are unique to Ar_Han0458.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment showed that the biosynthesis of phenylalanine,tyrosine and tryptophan was unique to the As2340 in,and the synthesis rate of tryptophan decreased from 00 h to 24 h and increased thereafter;while the decomposition rate of tryptophan increased from 0h to 48 h,and then decreased.In another hand,Ten DEGs were involved in the glutathione metabolism of Ar_Han0458,and the glutathione synthesis rate peaked at 24 h and 96 h.AS(alternative splicing)analysis indicated that the largest percentage of AS events in the two strains were SE(skipped exon),which accounted for 88.87% and 91.03% in As2340 and Ar_Han0458,and the following is alternative 3' splice site.While there was no RI(retained intron)in the two strain.The GO terms of the alternative spliced genes showed,in which the pigmentation was unique to As2340,and the cell proliferation was unique to Ar_Han0458,and the remaining functions differed to a maximum extent by 1 to 5 GO secondary items.The shared functions of Ar_Han0458 and As2340 mainly involve channel transport and substance transport,catalytic activity and protein substrates,while the unique functions of the two strains are mostly different subunits and substrate proteins.At the same time,the functions of the differentially spliced genes in both strains are aquaporins and copper transporters.The results of transcription factor analysis of SE showed that the microphone of the promoter region of recombinant yeast Ar_Han0458 was reduced by one compared to the original strain As2340,but in recombinant yeast,there are two new transcription factors related to the AP2/ERF region are added to Ar_Han0458.This study provides bioinformatics data for the further understanding of the mechanism of low energy ions on the metabolism and transcriptional regulation of eukaryotic microorganisms.It also provides a theoretical basis for further research on the regulation of secondary metabolites in ion beam recombinant strains.