Development And Characterization Of Clinical Grade Human Mesenchymal Stem Cell Banking And Manufacturing

Although mesenchymal stem cells(MSCs)based therapy has been considered as a promising tool for tissue repair and regeneration,the optimal cell source remains unknown.Umbilical cord(UC),dental pulp(DP)and menstrual blood(MB)are easily accessible sources,which make them attractive candidates for MSCs.The goal of this study was to compare the biological characteristics,including morphology,proliferation,anti-apoptosis,multi-lineage differentiation capacity and immunophenotype of UC-,DP-and MB-MSCs in order to provide a theoretical basis for clinical selection and application of these cells.As a result,all UC-,DP-and MB-MSCs have self-renewal capacity and multi-potentiality.However,the UC-MSCs seemed to have higher cell proliferation ability,while DP-MSCs may have significant advantages for osteogenic differentiation,lower cell apoptosis and senescence.These differences may be associated with the different expression level of cytokines,including vascular endothelial growth factor,fibroblast growth factor),keratinocyte growth factor and hepatocyte growth factor in each MSCs.Comprehensively,our results suggest we need choose the suitable MSCs to treat different disease.After comparing the biological characteristics of UC-,DP-and MB-MSCs,we choose UC-MSCs to study the clinic production.First,we use different culture system,to study the harvest time and cell cultivation,in the meanwhile,we also choose the right production system,we finally harvest the largest numbers of cells,the period is just suitable,and the cells kept in good state through the right culture condition.We also try to establish the quality assurance of stem cells drugs.