The Study On The Mechanical Properties Of Osteoblasts Based On Atomic Force Microscope

Cell mechanics is of great significance for understanding cell physiological and pathologic mechanisms as well as improving cell function.Atomic force microscopy(AFM)can perform mechanics tests of living cells with high sensitivity in physiological condition.Therefore,this paper researched cell mechanics of the cells in the process of osteogenic differentiation and those when seeded in different pure titanium material surface.This paper provided a new idea for the mechanism of osteogenic differentiation and the interaction between the cells and the surface of pure titanium.The main content of this paper is listed as follows:1.First analyze the significance of cell mechanics in the evaluation of osteogenic differentiation and the property bone integration of pure titanium materials.Then introduce the research status of microstructures of pure titanium surface and the application of AFM in the field of cell mechanics.2.Research the methods of the test of cell mechanics,introduce the basic principles of AFM and the test principles of the force curve.Put forward the elastic model which is suitable between the probe and the cells.Develop an algorithm theory detecting the contact point in the force curve.3.Select MC3T3-E1 cells as the research object of this project,introduce the culture process of cells,and point out the solutions of cell apoptosis and aging during cell culture.Then describe the experiment process of the cell mechanics measurement using AFM.4.Stimulate the MC3T3-E1 cells to osteogenic differentiation using chemical factor,then detect the process of cell differentiation,meanwhile test the mechanics properties of the cells at the same stage.Finally,analyze the changes of cell mechanics properties during the osteogenic differentiation.5.Prepare three kinds of titanium plates with different microstructures.Research the morphology,proliferation and the osteogenic differentiation of the MC3T3-E1 cells to evaluate the property of bone integration of titanium materials.Then perform the test of mechanical properties of the MC3T3-E1 cells to analyze the relationship between cell mechanics and structures of titanium plates to report the control effect oftitanium plate structure on cell.