Study On The Regulation Of Homologous Chromosome Synapsis By Centrosome Protein ZYG-1

Meiosis is a special type of cell division that occurs during the process of germ cell formation in sexually reproducing organisms.Meiotic abnormalities eventually lead to miscarriage or infertility.Accurate separation of homologous chromosomes requires that they first form stable interactions.In most species,the establishment of homologous chromosomal interactions is mainly coordinated through chromosome pairing and recombination.Previous studies have shown that the mechanism of cytoskeletal force driving chromosome movement in the pre-meiotic phase is highly conserved.In Caenorhabditis elegans,the pairing center interacts with the transmembrane protein SUN/KASH complex,which in turn is connected to cytoplasmic microtubuLes.MicrotubuLes drive chromosomal movement;a specific region called the pairing center can be linked to the nuclear membrane of the cell to regulate chromosomal movement,thereby promoting chromosome pairing and then initiating synapsis.In this paper,we use caenorhabditis elegans as model organisms found that centrosomes are involved in regulating homologous chromosomal synapsis.Our immunofluorescence experiments showed that the mutations of ZYG-1 and SPD-2caused the prolongation of leptotene/zygotene and the abnormal number of DAPI-stained bodies in the diakinesis stage.Using the synaptonemal complex proteins HTP-3 and SYP-2 as indicators,we found that the mutations of ZYG-1caused abnormalities in chromosomal synapsis.Ultimately,We concluded that the mutations of ZYG-1 caused the reduction of chromosomal dynamics,which in turn led to abnormalities in chromosomal synapsis.