Bioinformatics Analysis Of Alcohol Dehydrogenase 1A And Its Functional Analysis In Lung Cancer

Background:Cancer is an important contributor to the global burden of disease.Among them,lung cancer is the most frequently diagnosed neoplasia and represents the leading cause of cancer-related deaths worldwide.The human alcohol dehydrogenase 1(ADH1),which has three genotype ADH1 A,ADH1B and ADH1C,is an important member of the ADH gene family and.plays an important role in variety of solid tumors including lung cancer.Since there are scarce reported studies on ADH 1 As functions in cancer in the world literature,and in order to further understand the pathogenesis and progression of cancer,the role of ADH1A in various solid tumors,especially lung cancer was explored in this study.Methods and materials:We identified the mRNA expressed differences of ADH1A between various solid tumor tissues and normal tissues from The Genotype-Tissue Expression(GTEx)database and The Cancer Genome Atlas(TCGA)database.Paired tumor and normal tissues gathered from 38 tumor patients,and 5 male BALB/c mice tissues were collected and IHC assay was performed to identified the protein distribution profile of ADH1A.The main functions of ADH 1A in different solid tumors were analyzed by Bioinformatics analyses,include Co-expression analysis,principal component analysis,functional enrichment analysis,immune infiltration analysis,and survival analysis.We also predicted the up-stream regulation network and downstream protein-protein interaction(PPI)network of ADH1A in lung adenocarcinoma.Results:At the RNA level,ADH1A is highly expressed in normal hepatocytes and is expressed in most solid tumor tissues at a lower level than in the corresponding normal tissues,suggesting the potential and diagnostic and prognostic value of ADH1A.At the protein level,ADH1A is in the human alveolar,kidney,stomach,colon,rectum Liver tissues have different degrees of expression.The BALB/c mice could play a good role when establishing a mouse model of GC,ESCA,LIHC,and PAAD.We predicted three major conserved functions of ADH1A,including leukocyte migration function which could influence the immune response and prognosis of the immunotherapy,and constructed an upstream regulation of ADH 1A and a downstream protein network.Besides,we also explored the functional differences of ADH1A in lung adenocarcinoma and lung squamous cell carcinoma and its effect on overall survival.Conclusion:ADH1A has potential diagnostic value and potential prognostic value in various solid tumors.Our findings highlight new avenues for further investigation of the ADH1A biology process and provide a novel potential prognostic biomarker of immunotherapy.