Dynamic GWAS Study On Genetic Mechanism Of Three Bacterial Interactions And Gene Function Verification

Microorganisms interaction plays an important role in clinical disease treatment,ecosystem repair,pollution prevention and other fields.Studying the interaction between microorganisms is a prerequisite for us to make better use of microorganisms.Among them,the interaction between bacteria is in ecology and medical research has important practical value.In this study,Escherichia coli,Staphylococcus aureus and Pseudomonas aeruginosa were cultured under laboratory conditions,based on real-time fluorescent quantitative PCR and whole-genome resequencing to obtain phenotype data and genotype data,respectively,and applied dynamics based on growth parameters GWAS conducts bottom-up association analysis to study the genetic mechanism of bacterial interactions.In addition,based on the genetic mechanism of interaction,this study established experiments on the verification of the biological effects of the E.coli dna K and fts K genes based on previous studies,using traditional homologous recombination methods to knock out the target genes,combined with bacterial growth validates the bacterial growthrelated functions of the target gene.The study found that there are significant differences in the growth of the three bacteria under the monoculture and poly-culture.Combined with dynamic association analysis,the interaction mechanism is studied.Through gene annotation,all genes related to the growth and metabolism of the bacteria are located in the three bacteria.fli F,wca J,wca E,agp,etc.in E.coli;roc D,lip2,upp P,pck A,etc.in S.aureus;waa P,nqr B,etc.in P.aeruginosa.On the other hand,through biological function verification,it was found that the growth of the fts K gene knockout strain was significantly different from that of the original strain,and the growth of the knockout strain was significantly inhibited.In this study,through the successful positioning of dynamic GWAS,significant genes affecting bacterial interaction were obtained,and further attempts were made to verify the function of genes,and the experimental basis for verification was successfully established.This study is based on dynamic association analysis between bacteria,revealing the genetic mechanism of bacterial interaction,providing strong scientific theoretical support for disease treatment and ecological governance where multiple bacterial communities coexist,and laying the foundation for future research on more complex bacterial community interactions.