Studies Of Intestinal Delivery Of Probiotics

A large number of microorganisms colonize the mammalian intestine,which interacts with the body and plays a regulatory role in human health.When ingested in adequate amounts,probiotics can regulate the microecological balance and promote health.However,most probiotics have a poor resistance to adverse environmental conditions,resulting in tumultuous death during processing,storage and ingestion.Therefore,to explore the embedding and intestinal delivery technology of probiotics is of great vital significance for probiotics to resist the adverse environment,achieve intestinal delivery and improve human health.The main conclusions are as follows:(1)Monodisperse droplet generator(MDDG)was utilized to produce probiotics microcapsules with uniform particle size,which realized the efficient production of microcapsules.It was found that after 9 hours of cultivation in MRS medium,the spherical structure of multilayered probiotics microcapsules was still intact,and the number of probiotics increased by 2.06 log CFU/g.Meanwhile,the single-layer calcium alginate microcapsule disintegrated rapidly in simulated intestinal fluid.When digested for 1 hour,the release number of probiotics reached 8.23 log CFU/g,and the release number of multilayered microcapsules reached the corresponding value after 3 hours.In addition,after 12 days of storage at 4 ?,the number of live bacteria in the calcium alginate microcapsules coated with chitosan and acacia gum decreased by only 0.09 log CFU/g.The results showed that multilayered probiotics microcapsules had higher mechanical strength,which made improving the embedding rate of probiotics by short-term culture possible.At the same time,multilayered probiotics microcapsules prolonged the disintegration time in simulated intestinal liquid and showed higher storage stability.(2)Using soybean 7S globulin as a template,soybean 7S globulin/hydroxyapatite composite particles were successfully prepared by hydrothermal precipitation method.X-ray diffraction(XRD)and Fourier transform infrared spectroscopy(FTIR)analysis results showed that the crystallinity of composite particles with less protein content is higher.The particle morphology was observed by transmission electron microscopy(TEM)and scanning electron microscopy(SEM),and it was found that the mineralization process could be effectively regulated by adjusting the protein concentration in the solution during preparation,and the particle size and structure of composite particles could be changed.It was proved that hydroxyapatite has no inhibition on probiotics,which provided the possibility for it to be used as probiotics tablet excipients.(3)Soybean 7S globulin/hydroxyapatite composite particles were used as probiotics tablet excipients,probiotics tablet with high protein and calcium was prepared and the survival and release behavior of probiotics in tablets in simulated gastrointestinal fluid were studied.The results showed that the tablets made of 0.8 7S-HA had a certain intestinal sustained-release effect.As for the pressing pressure,when the compression pressure went up,the survival rate of probiotics in simulated gastric fluid gradually increased,but the survival rate during compression was reduced.The moderate ratio of raw materials(5:5)effectively protected probiotics during tablet pressing.However,the higher content of composite particles,the more stable the tablet structure,as a result,the tablets showed higher survival rate of probiotics in gastric liquid and the time of disintegration was prolonged in simulated intestinal fluid.