Synthesis And Properties Of Reactive Fluorescent Probes Based On Several Common Fluorescent Dye

In recent years,functional fluorescent dyes have been widely used in life and materials science.Among them,fluorescent chemical sensors have become important in the area of detection and analysis technologies because of their high sensitivity,strong selectivity,and easy detection.The role of the relationship is converted into a fluorescent signal.Common fluorescent dyes include:Coumarin,Fluorescein,Cyanine,Naphthalimide,Rhodamine,etc.This type of fluorescent dye has the advantages of high fluorescence quantum yield,good light stability,simple preparation and its structure is easily modified.Therefore,it is usually an ideal substrate for designing and synthesizing fluorescent probes,but in practical applications,the probe molecules have disadvantages such as poor water solubility and poor selectivity.In this paper,three reactive fluorescent probes were designed and synthesized based on hydrazine,hypochlorite and mercury ions.(1)The first part was based on the triphenylamine fluorophore to synthesize a fluorescent probe A with good water solubility and high selectivity for fluorescence colorimetric dual-channel detection of hydrazine hydrate.Probe A has triphenylamine as the fluorophore and malononitrile as the recognition group for the hydrated dioxime.The probe molecule enables dual colorimetric and fluorescent detection of hydrated combinations.When the hydrated combination was added to the probe A solution,the color of the probe solution changed from orange to colourless.At the same time,Probe A has also been successfully applied to the fluorescence imaging of hydrated hydrazines in Hela cells,and the probe molecule has very little toxicity to HeLa cells and has excellent biological compatibility.This will have potential application value in biomedicine in the future.(2)In the second part,the fluorescent probe B was designed and synthesized based on the phenothiazine fluorescent dye as the parent and hydrazine as the hypochlorite reaction site.Compared with the previously reported probes,the probe can realize hypochlorite ions with very quick response time.Probe B exhibit good selectivity and sensitivity and the calculated DL reached 1.35x10-7M.(3)In the third part,probe C was obtained by the nucleophilic addition reaction of furfural and ethanedithiol with fluorene as a fluorophore.The probe molecule was easily obtained by a one-step reaction.The sensing mechanism was the intermolecular charge transfer.Probe C exhibit good selectivity and sensitivity toward to Hg2+.