TiO₂ Based Nanomaterials And Its Apptlitions In Phosphorylated Protein Enrichment And Visible Light Sterilization

Titanium dioxide(TiO2)nano materials because of their unique surface adsorption and photoelectric catalytic properties,wide attention by the researchers,in the aspect of phosphoprotein enrichment and selective catalytic activity application prospect.However,the current commercialized TiO2 enriched materials have limited efficiency in capturing phosphorylated peptides.In addition,conventional TiO2 nanomaterials can only absorb ultraviolet light,but the absorption ability in the visible region is very low.Therefore,by modifying the nano Ti02 by surface structure or chemical modification,the enrichment ability of TiO2 and the absorption efficiency of visible light can be improved.Paper by improving solvent hot method,using tetrabutyl titanate as the monomer,adding hydrofluoric acid to control the crystal growth,crystal with high specific surface area of TiO2 nanoparticles were synthesized and TiO2 by doping Nd and Si nanomaterials modification,preparation a Nd-SiO2 @ TiO2(NST)functional nanomaterials.The structure and morphology structure of TiO2 nanometer crystal were studied by X-ray diffraction(XRD),TEM,we found that the preparation of TiO2 nanometer sheet with good rutile type and flaky morphology,and crystal structure is about 30 nm size,the particle size is less than commercial TiO2 particle 60 nm.When respectively with commercial commercial TiO2 nanorods and homemade TiO2 ball enrichment the simple β-casein the MALDI-TOF MS has rarely phosphated sites,and the TiO2 nanometer piece enrichment experiment about β-casein,found that there are seven phosphated sites,and the adsorption limit low to 2×10-9 M.Further enrichment β-casein and bovine serum albumin(BSA)of used for simulation of complex samples by the the TiO2 nanometer piece,five phosphated sites sites found in mass spectrogram.commercial TiO2 nanorods only one phosphoric acid sites at a very low concentration,while there are still two phosphate sites by Ti02 nanometer piece site,shows that the material of phosphorylated proteins with high specificity recognition and enrichment efficiency.The morphology and structure of NST functional nanomaterials were characterized By Raman spectroscopy,SEM and TEM images.XRD and Raman show that the crystal type of NST materials is anatase,and has the good crystallinity.SEM and TEM reflect the spherical morphology of the material,and the particle size is about 28 nm,which is less than commercial TiO2(P25).The forbidden band width of NST and P25 calculated by ultraviolet spectrogram is 2.96 eV and 3.20 eV,respectively,and the change of energy level structure is that NST generates the visible light response capability.It can be seen from infrared spectrogram and physical adsorption data that NST has a larger specific surface area and more indicating hydroxyl,and it is known that NST has better adsorption and photogermicidal effect.In this experiment,Escherichia coli(E.C)were selected for the photocatalysis sterilization experiment.MALDI-TOF MS showed that the sterilization efficiency was 100%after 120 min of simulated sunlight.