Study On Enzymatic Activity Of Pt Hollow Nanodendrites And Its Application For Wound Disinfection

Bacterial infections pose a large public health concern due to the rapid emergence of antibiotic resistance.Traditional antibiotics and antibacterial agents have been unable to cope with increasing bacterial infections.Therefore,the development of novel antibacterial agents is highly desirable.Hydrogen peroxide was widely used in the practical treatment of bacterial infections as an antibacterial agent at present.The high concentration of H₂O₂ is harmful to healthy cell and tissue.Therefore,it is important to improve the antibacterial activity and lower the concentration of H₂O₂ for wound disinfection.The ability of nanozymes to convert H₂O₂ to hydroxyl radicals that are more toxic to bacteria has been reported.Based on this research background,this paper optimized enzyme-like activity by regulating the structure of nanomaterials,and applied to H₂O₂ in disinfection.Due to high catalytic activity and well-controlled shape,size and structure,we design and synthesis of Pt hollow nanodendrites with Pd cubes as precursors.This specific structure can significantly increase the surface atomic ratio and effectively improve its enzyme-like catalytic activity for enhances the antibacterial activity of H₂O₂and wound disinfection.The main contents are listed as follow:1.Pt hollow nanodendrite were designed and prepared via seed-mediated growth and selective chemical etching.The formation process and structure of dendrites were explored by transmission electron microscopy,energy dispersive X-ray spectroscopy,UV-visible infrared spectrophotometer.2.Peroxidase-like activities of Pt hollow nanodendrites were evaluated by TMB oxidase with H₂O₂.The V_maxvalue for Pt hollow nanodendrites was almost 55 times higher than its precursor by kinetic assays.Electron spin resonance spectroscopy and terephthalic acid fluorescence experiments were used to study the catalytic reaction mechanism.3.The antibacterial capacity of H₂O₂/Pt hollow nanodendrites were assessed by in actual wound infections.Meanwhile we evaluated the biosafety of Pt hollow nanodendrites was evaluated by in vitro and in vivo experiments.