| Letinous edodes polysaccharide(lentinan,LNT)is a toadstool ke Letinous edodes polysaccharide(Lentinus edodes)of mycelium,after deep liquid culture the extraction of polysaccharide.Letinous edodes polysaccharide is a kind of more than 10 monosaccharides by glycosidic bond connection of macromolecule polymers,with complex chemical structure of macromolecular material.Letinous edodes polysaccharide tablets are Letinous edodes polysaccharide as the main component of system has some features of biochemical drugs,Letinous edodes polysaccharide piece can be triggered by host immune system cells and the humoral immune system,enhance the host antiviral,antitumor and antibacterial,used for various diseases caused by low immune function,and chronic viral hepatitis,liver treatment;Can also be used as a secondary tumor chemotherapy drugs enhance immunity,tumor patients to curb the development of tumor,improve the quality of life of patients.In this paper,we study with Letinous edodes monosaccharide composition of polysaccharides and quality analysis,and production of Letinous edodes polysaccharide with Letinous edodes polysaccharide materials Letinous edodes polysaccharide of monosaccharide composition,heavy metals and mineral nutrient content and molecular weight distribution were studied systematically,and raw material of Letinous edodes polysaccharide initially the inhibition of tumor cell proliferation,comprehensive results four aspects to evaluate the quality of the raw material of Letinous edodes polysaccharide analysis,and respectively in the Letinous edodes polysaccharide in the verification,the results of Letinous edodes polysaccharide between raw material and preparation compares,analyses the reason of the differences between the raw materials and preparations,in order to further improve and rationalization of Letinous edodes polysaccharide production technology provide material foundation,further improve the understanding of Letinous edodes polysaccharide existing.Thesis mainly for the following research:(1)Letinous edodes polysaccharide determination of monosaccharide composition analysis method and analysis determination of the samples.By optimizing the hydrolysis,derivative and chromatographic conditions,the PMP derivative-ultra high performance liquid chromatography(HPLC)method for Letinous edodes polysaccharide materials and Letinous edodes polysaccharide of monosaccharide composition analysis.The results showed that the Letinous edodes polysaccharide materials and Letinous edodes polysaccharide in the monosaccharide composition of mannose,glucose and galactose;Using sugar nitrile acetylated derivatives-gas chromatography raw material of Letinous edodes polysaccharide were analyzed.The results showed that the Letinous edodes polysaccharide main monosaccharide composition of raw material for mannose,glucose and galactose.Suspected to contain a small amount of xylose and rhamnose,fucose,Arabian sugar,there is interference for chromatographic peak,for further confirmation.In order to further confirm its monosaccharide composition,USES the high sensitivity of ion chromatography pulse ampere detector in the raw material of Letinous edodes polysaccharide the composition of monosaccharide do further analysis,and tests this with the Letinous edodes polysaccharide piece.The results showed that the Letinous edodes polysaccharide material ion chromatograms response,no other monosaccharide components in Letinous edodes polysaccharide can be drawn from the raw material of monosaccharide composition of mannose,glucose and galactose.Letinous edodes monosaccharide composition of polysaccharides of mannose,glucose and galactose.Three detection methods,gas chromatography and ion chromatography method is further evidence that the ultra high performance liquid chromatography(HPLC)method to optimize the rationality and accuracy of the experimental set up of the ultra high performance liquid chromatography(HPLC)method suitable for analysis of Letinous edodes monosaccharide composition of polysaccharides.(2)the experimental set up Letinous edodes polysaccharide of heavy metals and mineral nutrient content in the raw material and tablets,the determination methods of using inductively coupled plasma mass spectrometer based on Letinous edodes polysaccharide and Letinous edodes polysaccharide analysis of raw materials,raw materials in the detection of heavy metal elements are in line with the national food safety standards of food contaminants in the limits of the requirement,Letinous edodes polysaccharide of A1 element content on the high side,79.4 mg/kg.This part of the research for the Letinous edodes polysaccharide of heavy metals and mineral nutrient content in the raw material test provides a feasible daily detection method.(3)the Letinous edodes polysaccharide content determination of molecular weight.High gel chromatography column liquid phase methods for the determination of polysaccharide separation,through Letinous edodes polysaccharide molecular weight determination of the raw material and Letinous edodes polysaccharide as a result,the analysis of the distribution coefficient of raw material and tablets,it can be seen from the results,Letinous edodes polysaccharide three kinds of raw materials of ABC heavy molecular weight difference is not obvious,but the number average molecular weight difference is significant,lead to Letinous edodes polysaccharide material smaller distribution coefficient of B,C,explain the sample homogeneity is good,the distribution is uniform,Letinous edodes polysaccharide material distribution coefficient greater than 10.0,shows the sample homogeneity is poorer,uneven distribution.Letinous edodes polysaccharide the distribution coefficient of smaller,explain the sample homogeneity is good,the sample distribution more uniform.(4)Letinous edodes polysaccharide on inhibition of tumour cells,a preliminary study on the determined by MTT method with Letinous edodes polysaccharide raw materials and dried Letinous edodes polysaccharide inhibition of 7721 liver cancer cells,respectively by the enzyme standard instrument measurement results with Letinous edodes polysaccharide material and Letinous edodes polysaccharide,shows the vitality of Letinous edodes polysaccharide material are 55.9%,48.7%and 55.9%respectively,Letinous edodes polysaccharide energy 51.3%,shows that Letinous edodes polysaccharide and Letinous edodes polysaccharide piece of raw material has inhibitory effect on the growth of liver cancer cells.All inspection items according to the above research,correlation analysis,it can be seen that both Letinous edodes polysaccharide material and Letinous edodes polysaccharide are shortcomings in one area,manufacturers in the production tips can be targeted to the Letinous edodes polysaccharide increase with raw material and tablets respectively,experiments of Letinous edodes polysaccharide chemical structure,biological activity and quality analysis to provide important reference basis.And to make systematic Letinous edodes polysaccharide material basis for quality control,ensure the effectiveness of the Letinous edodes polysaccharide piece,safety and quality control.To ensure safe,effective,stable and controllable Letinous edodes polysaccharides applied to clinical. |
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