Influencing Factors And Mechanism Of Kichen Waste Fermentation

Food waste is one of the ship's organic solid wastes and is highly susceptible to spoilage.If not handled in time,it will pose a great threat to the living environment.At the same time,kitchen waste is rich in organic components and has huge potential for fermenting production.Based on the above reasons,this article makes use of the kitchen waste in Harbin as the research object,innovatively coupling the fermentation kinetics equation and response surface methodology to study the pretreatment methods of kitchen waste and the influence factors of fermentation;and through high-throughput sequencing Means to study the microbial community succession before and after fermentation.In the pretreatment scheme,thermal pretreatment and alkali pretreatment were selected for the study.Under the condition of thermal pretreatment,using the thermal pretreatment temperature and treatment time for orthogonal experiment and energy consumption calculation,it was found that when the pretreatment quality exceeds 85 g,the substrate treatment at 60° C.for 50 min can guarantee the gas production effect.Lower processing power consumption.Under alkali pretreatment conditions,the alkali concentration and treatment temperature were orthogonal to each other.The results showed that the kitchen waste was treated under the environment of 30 g/L NaOH at-5°C,and the productivity was the best.The fermentation temperature,initial pH,and inoculation ratio were selected for the study of the influencing factors.According to the experiment of fermentation temperature,the fermentation was carried out in an environment of 55°C,and the productivity was the best.According to the initial pH test,the increase of the initial pH can effectively increase the gas production.The initial pH of 8.5 is the best in the test range.In the study of the proportion of inoculation,when the proportion of inoculation was 3/1,the effect of the kitchen waste on the fermentation capacity was the best.In the end,the high-throughput sequencing analysis was performed on the initial sludge samples and the final samples of thermal pretreatment and alkaline pretreatment.The microbial diversity in the initial sludge samples was good,which is suitable as an inoculum for microbial screening.After heat pretreatment,the microbial diversity of the two groups was reduced,and the proportion of Bacteroides and Enterobacter increased.More conducive to the production of hydrogen.The diversity of the microorganisms was lowest in the alkali-pretreated final samples,but it was shown that the four main microorganisms that were screened played a major role in the fermentation.The results of this study have a high reference value for improving the energy conversion efficiency of kitchen waste,and provide a theoretical basis for the regulation of fermentation production process.