Rabies Vaccine Preparation Process Optimization Research

Rabies is an acute infectious disease of the central nervous system caused by rabies virus infection,and there is no effective drug treatment.Once the disease occurs,the mortality rate is almost 100%.Rabies can only be prevented by injection of rabies vaccine.At present,the process for preparing whole virus inactivated vaccines includes preparation of virus harvest liquid by culture cells,virus harvest solution concentrated,inactivated,purified,prepared and freeze-dried.The widely used vaccine strains in China include rabies virus isolated from abroad(PV strains,PM strains,Flury strains)and rabies virus isolated from china(aG strains,CTN strains).The main stromal cell included primary hamster kidney cells,Vero cells,MRC-5 human diploid cells.Upstream production process includes roller bottle culture,cell factory culture,bioreactor culture.The purification process was mainly purified by molecular exclusion,ion exchange,purification.This paper mainly work for rabies vaccine strain RNA sequencing and analysing,confirming with other vaccine strains and CVS rabies strains on genetic distance,experiment on culture conditions of rabies virus bioreactor with high microcarrier concentration,preliminary purification conditions experiment and safe and effective test.The preparation process of rabies vaccine for human being was determined,which consisted of rabies virus strains isolated form china,vrius culture method for high medium density bioreactor with commercially available medium,purification method without DNA enzyme treatment.1.RNA extraction,sequencing and sequence analysis of rabies vaccine virus strain.2.Appendorf NBS Cellgen 310 7.5L bioreactor used for rabies virus culture conditions selecting.3.rabies vaccine inactivated and purification production practice4.Preliminary safety evaluation of rabies vaccine samplesThrough this research,the genetic lineage situation of rabies virus is confirmed,rabies virus culture and purification of rabies vaccine is determined,and preliminary verification is conducted.The preparation process of rabies vaccine is determined.It provides the direction and basis for optimizing the process of rabies vaccine.