Rational Design And Application Of Novel Plasmonic Nanoprobes For The Selective Sers Detection And Therapy Of Cancer Cells

Malignant tumors(cancer)is a terrible disease in today’s society.The fear is that the current medical methods are difficult to achieve early detection,early diagnosis,early treatment of the "three early" policy,in most cases it will be diagnosed only in the middle to late stage,which greatly reduces the cure rate of patients.Therefore,the importance of early diagnosis of cancer cannot be ignored.In recent years,it has been found that circulating tumor cells can be used effectively as a "liquid biopsy" sample for evaluating cancer occurrence.Accurate analysis of this can not only accurately determine the occurrence or absence of cancer,but also has a great guiding significance for the evaluation of prognosis.Design of drug delivery systems represents a major strategy to achieve specific targeting delivery,controlled release of drugs and high cell-membrane permeability,and facilitate cellular internalization.Nanomaterial drug carriers are especially attractive due to their advantageous properties.On the other hand,in recent years,an outstanding metal-directed self-assembly strategy has been described for the fabrication of protein crystals.Inspired by the construction principles of MOF and metal-directed protein crystals,we propose here the rational engineering of drug crystalline frameworks(DCFs).Surface-enhanced Raman spectroscopy(SERS)can accurately detect the analytes in complex systems due to its non-destructive test conditions,rapid and sensitive detection,and sharp spectral peaks.And it has been widely used in biological analysis and tumor imaging.1.Nanogold is in situ synthesized on the surface of nano magnetic Fe304,and the Raman signal molecule and the tumor targeting ligand are bonded to the surface to form a magnetically separable SERS immuno-trapping probe.2.Designing and synthesizing a nano-drug delivery system based on a protein framework to enable sustained release of drugs and specific tumor-targeted endocytosis based on the principle of pH sensitivity3.Surface-enhanced Raman spectroscopy was used to dynamically monitor the sustained-release process of endocytosis of nano-drug sustained-release system in tumor cells to determine the sustained-release process of the system.