| Immunization is an effective way to prevent and treat diseases.Individual vaccine delivery is easily degraded by proteases to reduce immunogenicity.Nanoparticles as vaccines delivery carriers can enhance the immune response,particularly the cellular immunity to against infectious viruses and malignant tumors.Nevertheless,the applications of some nanoparticles have been limited because of non-biocompatibility and the high cost and so on.Metal-organic frameworks?MOFs?are porous materials with crystal structures.MOFs are constructed by bridging metal ions or clusters with organic ligands.Because of their high loading capacity and simple preparation,they have been widely used for biomedical applications.In the study,we use Fe-MIL-101-NH2?MOF?as vaccine delivery carriers for immunization.1.Physico-chemical characterizations and biocompatibility of MOF.MOF is synthesized by hydrothermal method,then physico-chemical characterizations and biocompatibility were evaluated.As a result,positive charged MOF is octahedron crystal with averge particle size 553 nm.At low concentrations,its blood compatibility is good and cytotoxicity is weak.2.The MOF co-loads the antigen model ovalbumin?OVA?and an immune adjuvant?unmethylated cytosine-phosphate-guanine oligonucleotide CpG?for subcutaneous immunization.In this work,OVA is covalently linked to MOF through disulfide bonds?MOF-S-S-OVA?.MOF-S-S-OVA is loaded with CpG through electrostatic adsorption.MOF-S-S-OVA@CpG is physicochemically characterized and the effect of immune response is examined by subcutaneous inoculation.The results showed that positive charged MOF-S-S-OVA@CpG with averge particle size 574 nm induces a significant high-level antigen-specific IgG antibody,increases the secretion of cytokines IFN-?,IL-4 and TNF-?,increases the proliferation of spleen cells,and promotes differentiation of memory T cells and CTL lethality to target cells.The above results show that MOF can be a potential vaccine carrier. |
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